Evaluation of steam explosion pre-treatment for enzymatic hydrolysis of sunflower stalks

Enzyme and Microbial Technology
Encarnación RuizEulogio Castro

Abstract

Sunflower stalks, a largely available and cheap agricultural residue lacking of economic alternatives, were subjected to steam explosion pre-treatment, the objective being to optimize pre-treatment temperature in the range 180-230°C. Enzymatic hydrolysis performed on the pre-treated solids by a cellulolytic complex (Celluclast 1.5L) and analysis of filtrates were used to select the best pre-treatment temperature. Temperature selection was based on the susceptibility to enzymatic hydrolysis of the cellulose residue and both the cellulose recovery in the solid and the hemicellulose-derived sugars recoveries in the filtrate. After 96h of enzymatic action, a maximum hydrolysis yield of 72% was attained in the water-insoluble fiber obtained after pre-treatment at 220°C, corresponding to a glucose concentration of 43.7g/L in hydrolysis media. Taking into account both cellulose recovery and hydrolysis yield, the maximum value of glucose yield referred to unpretreated raw material was also found when using steam pre-treated sunflower stalks at 220°C, obtaining 16.7g of glucose from 100g of raw material. With regard to the filtrate analysis, most of the hemicellulosic-derived sugars released during the steam pre-treatment were in oligom...Continue Reading

References

Jun 9, 2001·Enzyme and Microbial Technology·C TengborgG Zacchi
Jul 31, 2002·Bioresource Technology·Sanjeev K SharmaHarmeet S Grewal
May 2, 2003·Applied Biochemistry and Biotechnology·Maria José NegroMercedes Ballesteros
Nov 5, 2003·Applied Microbiology and Biotechnology·T W Jeffries, Y-S Jin
Dec 14, 2004·Bioresource Technology·Nathan MosierMichael Ladisch
Aug 19, 2006·Applied Biochemistry and Biotechnology·Encarnación RuizEulogio Castro
Mar 21, 2007·Bioresource Technology·S CaparrósL Jiménez

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Citations

Nov 13, 2010·Journal of Industrial Microbiology & Biotechnology·Jaime Saucedo-LunaJesus Campos-Garcia
Aug 12, 2009·Applied Biochemistry and Biotechnology·Xun HePingkai Ouyang
Sep 21, 2013·Applied Biochemistry and Biotechnology·Namita BansalSanjeev Kumar Soni
May 21, 2014·Journal of Environmental Health Science & Engineering·Jagdish GabhaneSatish Wate
Apr 9, 2014·Biotechnology for Biofuels·Dina JabbourJonathan D Walton
Jul 2, 2014·Bioresource Technology·Raquel Martin-SampedroMaria E Eugenio
Jan 13, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Karen Wilson, Adam F Lee
Nov 21, 2014·Chemical Reviews·Ana R C MoraisRafał Bogel-Łukasik
Jul 17, 2010·Bioresource Technology·Aloia RomaníJuan Carlos Parajó
Jun 15, 2010·Journal of Bioscience and Bioengineering·Yuya YamashitaYoshitoshi Nakamura
May 1, 2015·Bioprocess and Biosystems Engineering·Priyadharshini RamachandranJung-Kul Lee
Jul 12, 2011·Bioresource Technology·Xuebin LuIrini Angelidaki
Apr 25, 2013·Bioresource Technology·Chan-Duck JungKyung-Sik Hong
May 28, 2013·Bioresource Technology·Encarnación RuizEulogio Castro
Dec 22, 2009·Bioresource Technology·Carlos Ricardo SoccolFernando Araripe Gonçalves Torres
Oct 16, 2015·Bioresource Technology·L V Daza SernaC A Cardona Alzate
Oct 21, 2016·Applied Microbiology and Biotechnology·Laura Capolupo, Vincenza Faraco
Oct 20, 2015·New Biotechnology·Roberto A AgudeloJohann F Görgens
Mar 28, 2009·International Journal of Molecular Sciences·Mohammad J Taherzadeh, Keikhosro Karimi
Jan 29, 2016·Applied Biochemistry and Biotechnology·M KharinaT Gorshkova
Jun 11, 2019·Frontiers in Chemistry·Alexandra SchindlAnna K Croft
Feb 14, 2017·Biotechnology for Biofuels·Thomas AuxenfansGabriel Paës
Dec 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Malte Jörn KrafftBodo Saake
Jun 24, 2008·Journal of Agricultural and Food Chemistry·Bibin Mathew CherianSabu Thomas

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