Evaluation of stearate-graphite paste electrodes for chronic measurement of extracellular dopamine concentrations in the mammalian brain

Pharmacology, Biochemistry, and Behavior
C.D. Blaha

Abstract

Chronoamperometric procedures, in combination with pharmacological treatments, were used to verify whether stearate-modified graphite paste recording electrodes (SGEs) could measure basal extracellular dopamine (DA) concentrations in the striatum of awake rats over a 3-week period of implantation. Baseline chronoamperometric signals were unaffected by systemic injections of ascorbate (AA) or the monoamine oxidase inhibitor pargyline, or by intraventricular infusions of the AA degrading enzyme AA-oxidase. In contrast, systemic injections of d-amphetamine or nomifensine increased, and gamma-butyrolactone decreased, the signal in a reproducible fashion over a similar test period. In addition, 6-hydroxydopamine lesions of the nigrostriatal DA pathway attenuated the ability of d-amphetamine to increase, and gamma-butyrolactone to decrease, the chronoamperometric signal. In separate studies, reverse microdialysis, performed with dialysis probes implanted directly adjacent to SGEs in the striatum, permitted the assessment of electrode selectivity, sensitivity, response linearity, and detection limits to DA. Perfusion of the probe with normal Ringer solution (5 microliters/min) decreased the baseline chronoamperometric signal by 10 nA....Continue Reading

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Citations

Jan 5, 2002·Behavioural Brain Research·Patricia Di CianoAnthony G Phillips
Oct 27, 2004·Naunyn-Schmiedeberg's Archives of Pharmacology·Manfred GerlachPeter Riederer
Apr 20, 2010·Pharmacology, Biochemistry, and Behavior·Caterina MarcangionePaul B S Clarke
Sep 27, 2005·Brain Research·Stewart D ClarkOlivier Civelli
May 2, 2012·Journal of Neuroscience Methods·Yuexiang Wang, Adrian C Michael

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