Evaluation of sterilization methods following contamination of hamstring autograft during anterior cruciate ligament reconstruction.

Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
Matthew J PlanteNicola A DeAngelis

Abstract

Inadvertent contamination of the hamstring autograft during ACL reconstruction is infrequent, but can result in significant complications. The purpose of this study is to evaluate bacterial contamination of hamstring autografts dropped onto the operating room floor and methods of graft decontamination. Hamstring tendons were harvested from patients. Excess tendon not used in the ACL procedure was divided into 6 segments. Segments were assigned to 6 groups (A through F, N = 30 in each group): group A: uncontaminated graft immediately postharvest (control), group B: graft dropped onto the floor (5 s), group C: graft dropped onto the floor (15 s). grafts in groups D to F were dropped onto floor for 15 s then rinsed with saline (group D), bacitracin solution (group E) or chlorhexidine 4 % solution (group F) for 3 min. All grafts were sent to the microbiology laboratory for anaerobic and aerobic cultures. Cultures were positive in 23 % of graft segments from group A (7/30), 33 % of grafts from group B (10/30), 23 % from group C (7/30), 30 % from group D (9/30) and 3 % from both group E (1/30) and group F (1/30). Sixteen unique organisms were identified, with Staphylococcus aureus as the most common isolate. Grafts rinsed in either b...Continue Reading

References

Jan 1, 1991·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·D E CooperR F Warren
Apr 6, 2000·The American Journal of Sports Medicine·T BurdW C Allen
May 10, 2000·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·M E MolinaJ C Delee
Oct 18, 2002·AORN Journal·Deborah M Boni, George E Herriott
Jun 27, 2003·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·P Díaz-de-RadaJ R Valentí
Dec 6, 2005·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Rolando IzquierdoChristopher S Ahmad
Mar 14, 2007·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Charles B Pasque, Timothy M Geib
Apr 13, 2007·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Jonathan MarraleFares S Haddad
May 1, 2007·The American Journal of Sports Medicine·David R GuelichBubba Wilson
Apr 3, 2008·The Journal of Bone and Joint Surgery. American Volume·Michael E HantesKonstantinos N Malizos
Apr 30, 2008·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Jeffrey TumanJoseph M Hart
Aug 9, 2008·The Journal of Knee Surgery·Richard D Parker, Steven D Maschke
Mar 12, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V S M SaegemanJ Verhaegen
Jun 19, 2009·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Iosif GavriilidisHans H Paessler
Dec 19, 2009·Clinical Orthopaedics and Related Research·Sameer H NagdaStephen J Lombardo
Apr 7, 2010·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Clare L ArdernJulian A Feller
May 4, 2011·The Journal of the American Academy of Orthopaedic Surgeons·Walter R Shelton, Bryan C Fagan

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Citations

Nov 25, 2014·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Daniel Pérez-PrietoJoan Carles Monllau
Feb 4, 2014·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Moin KhanOlufemi R Ayeni
Nov 5, 2013·The Journal of the American Academy of Orthopaedic Surgeons·Edwin R CadetBrian M Schulz
Mar 17, 2015·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Michael PheganChristopher J Vertullo
Jan 23, 2016·The American Journal of Sports Medicine·Abdulaziz Z AlomarFawzi F Aljassir
Nov 26, 2015·Sports Health·Alberto GobbiJohn Lane
Jun 12, 2013·The American Journal of Sports Medicine·Gregory B MaletisTadashi T Funahashi
Jul 25, 2017·The American Journal of Sports Medicine·Anchal BansalRobert H Brophy
Aug 31, 2017·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Abdulaziz Z AlomarAli M Somily
Oct 15, 2019·The Journal of Bone and Joint Surgery. American Volume·J E BaronR W Westermann
Jan 19, 2019·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Jan-Hendrik NaendrupThomas R Pfeiffer
Mar 10, 2017·The Physician and Sportsmedicine·Vidushan NadarajahMehul R Shah
Aug 1, 2019·Revista Brasileira De Ortopedia·Eduardo Frois TemponiLúcio Honório de Carvalho Júnior
Jan 23, 2019·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Christoph OfferhausJürgen Höher
Sep 10, 2020·Revista Brasileira De Ortopedia·Roberto Cunha LucianoDyego Vilela Luciano
Oct 29, 2020·Journal of Orthopaedic Trauma·Seyed Mohammad Javad MortazaviAli Shahabinezhad
Nov 2, 2020·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Joseph D LamplotRussell F Warren

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