PMID: 9183002May 1, 1997Paper

Evaluation of subunit truncation and the nature of the spacer for single chain human gonadotropins

European Journal of Biochemistry
J C HeikoopP D Grootenhuis

Abstract

Three single chain gonadotropins were designed based on the three-dimensional-structure of human choriogonadotropin and structure/activity relationships of the glycoprotein hormones. In each single chain, the C-terminal end of the human choriogonadotropin beta subunit is connected via Ser-Gly repeats to the N-terminal end of the alpha subunit. In addition, two of the single chains have truncated subunits. The three mutants were expressed in CHO cells. In vitro binding of two of the three mutants to the human lutropin/choriogonadotropin receptor was found to be comparable to wild-type lutropin. In contrast, both the receptor binding and the in vitro bioactivity of the mutant with truncated alpha and beta subunits in which the beta:26-110 disulphide bond cannot be formed, are lowered relative to wild-type lutropin. The fact that this mutant still displays biological activity shows that the seat-belt arrangement proposed by Isaacs and coworkers [Lapthorn, A. J., Harris, D. C., Littlejohn, A., Lustbader, J. W., Canfield, R. E., Machin, K. J.. Morgan, F. J. & Isaacs, N. W. (1994) Nature 369, 455-461] is important but not essential for receptor binding and biological activity in the context of single chain gonadotropins. Single chain...Continue Reading

References

Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Dec 8, 1995·Science·B LiB C Cunningham
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T SugaharaI Boime
Jun 9, 1994·Nature·A J LapthornN W Isaacs
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·A C Braisted, J A Wells
May 1, 1996·Molecular Human Reproduction·W OlijveP M van Wezenbeek

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Citations

Apr 2, 1999·European Journal of Biochemistry·J C HeikoopP D Grootenhuis
Apr 25, 2000·Nucleic Acids Research·E B VervoortM H Linskens
Sep 29, 2011·International Journal of Molecular Sciences·Anton P Le BrunJeremy H Lakey
Apr 29, 2006·Molecular and Cellular Endocrinology·Ruth Braw-TalDavid Ben-Menahem
Jan 8, 2017·General and Comparative Endocrinology·Hanna B Buechi, Jamie T Bridgham
Mar 4, 2000·Endocrine Reviews·M P RoseA H Balen
Mar 30, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M H LinskensJ C Heikoop
Apr 15, 2000·Methods : a Companion to Methods in Enzymology·P NarayanD Puett
Jul 10, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·M P Rose

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