Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0

Journal of Structural Biology
Po-Lin ChiuThomas Walz

Abstract

The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.

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Citations

Nov 2, 2016·Computational and Structural Biotechnology Journal·Slavica Jonić
Sep 4, 2018·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Rebecca S DillardElizabeth R Wright
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Apr 6, 2021·Journal of Structural Biology: X·J Ryan FeathersJ Christopher Fromme
Jun 27, 2021·The Journal of Biological Chemistry·Chloe Du TruongRajiv Kumar
Feb 23, 2020·Journal of Chemical Information and Modeling·Michael A Cianfrocco, Elizabeth H Kellogg

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