Evaluation of supercritical fluid chromatography coupled to tandem mass spectrometry for pesticide residues in food

Journal of Chromatography. a
Víctor CutillasAmadeo R Fernández-Alba

Abstract

Supercritical fluid chromatography coupled to triple quadrupole mass spectrometry has been evaluated for pesticide residues in food. In order to check its advantages and limitations it was developed a method to identify and quantify 164 pesticides in three different matrices (tomato, orange and leek). A carbon dioxide gradient with methanol (containing 1 mM ammonium formate) was used allowing a flow rate of 1.5 mL/min that made the total run time of 12 min without any problem of overpressure. Addition of a post column flow 150 μL/min of Methanol with ammonium formate/formic acid was necessary to improve the ionization. The matrix effect study revealed that the percentages of pesticides with irrelevant matrix effect (suppression lower than 20%) was 99% in tomato, 87% in orange and 62% in leek, whereas significant suppression (higher than 50%) was not found in tomato and only 1% of the compounds in orange and 3% in leek.These results compare favorably with that typically obtained in LC-MS/MS. The absence of water in the mobile phase, also provided some important advantages regarding LC-MS/MS as (i) higher retention of polar compounds in the column, which elute with high sensitivity and good peak shape and (ii) a general increase ...Continue Reading

Citations

Jul 28, 2018·Analytical and Bioanalytical Chemistry·Caroline West
Jun 29, 2018·Analytical and Bioanalytical Chemistry·Steven J Lehotay, Yibai Chen
Mar 23, 2019·Critical Reviews in Analytical Chemistry·Li-Xia LiuXue-Song Feng

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