Evaluation of the action of cholinergic agonists and cyclic guanosine monophosphate (c'GMP) on histamine release from purified rat mast cells

Immunopharmacology
C J HannaR R Schellenberg

Abstract

Results from previous studies providing conflicting data regarding the effects of cholinomimetic drugs and cyclic 3',5'-guanosine monophosphate salts (cyclic GMP) upon the release of histamine from various tissues. In the present investigations, highly purified rat peritoneal mast cells were incubated with a wide range of concentrations of carbachol, acetylcholine, and cyclic GMP in the absence and presence of the histamine releasing agents compound 48/80 and concanavalin A (Con A). Neither carbachol nor acetylcholine (10(-13) to 10(-4) M) alone caused rat mast cells to secrete histamine. Furthermore, when these drugs were combined with Con A (Con A 1 and 10 microgram/ml, carbachol 10(-13) to 10(-4) M) and compound 48/80 (compound 48/80 0.2 microgram/ml) acetylcholine 10(-13) to 10(-4) M), no enhancement of histamine release was observed. Cyclic GMP (8-bromo and dibutyryl salts) failed to elicit histamine release from rat mast cells over the concentration range 10(-7) to 10(-3) M. As well, cyclic GMP did not enhance Con A (10 microgram/ml)-induced histamine release. In fact this substance inhibited the release by as much as 15% at 10(-3) M concentrations. The present data suggest that neither cholinomimetic drugs nor cyclic GMP...Continue Reading

References

Jan 1, 1975·Annual Review of Pharmacology·M Kaliner, K F Austen
Jun 1, 1976·European Journal of Pharmacology·M C Holroyde, P Eyre
Jun 8, 1978·Nature·R FantozziT Bani-Sacchi
Jan 1, 1976·International Archives of Allergy and Applied Immunology·J F Burka, P Eyre
Sep 1, 1972·The Journal of Experimental Medicine·M KalinerK F Austen
Nov 1, 1972·The Journal of Clinical Investigation·E Gillespie, L M Lichtenstein
Aug 9, 1973·The New England Journal of Medicine·M KalinerK F Austen

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