PMID: 9661301Jul 14, 1998Paper

Evaluation of the allergenicity of spore and mycelia extracts of Pisolithus tinctorius

Revista Do Instituto De Medicina Tropical De São Paulo
J K ChavascoL C do Nascimento

Abstract

The antigenic and allergenic chemical analysis of spore and mycelia extracts of Pisolithus tinctorius was carried out. The spores were collected from basidiocarps in plantations of Eucalyptus spp and the mycelia from culture in MNM medium. With basis on the fungus growth curve, the mycelia masses were obtained after 10, 20, 30, and 40 days of incubation, which correspond, respectively, to the beginning, middle and end of the log phase, and beginning of the decline phase. The mycelia masses, together with the spores, were submitted to the action of three extractors (Coca, Tris-HCl, and ammonium bicarbonate). The contents of carbohydrates and proteins were determined. The SDS-PAGE electrophoretical analysis revealed separate fractions in these extracts, besides common fractions, in function of cultivation time and extraction methods. The selected extracts for the allergic tests were the ones with the highest number of fractions. The prick-tests were conducted in 374 patients--rural workers, eucalyptus plantation workers, and college students. The positivity to the "prick test" with the antigenic extract of P. tinctorius was, respectively, 3.78%, 28.20% and 6.40%. Most prick-test positive patients (82.75%) also presented symptoms ...Continue Reading

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Citations

Jul 6, 2014·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Geusa Felipa De Barros BezerraMaria do Desterro Soares Brandão Nascimento

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