Evaluation of the antiproliferative activity of the leaves from Arctium lappa by a bioassay-guided fractionation.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Fabio Bahls MachadoJoão Carlos Palazzo de Mello

Abstract

Arctium lappa L. (Asteraceae) is used in folk medicine around the World, and shows several kinds of biological activity, particularly in vitro antitumor activity in different cell lines. This study evaluated the antiproliferative activity of the crude extract, semipurified fractions, and isolated compounds from the leaves of A. lappa, through bioassay-guided testing in Caco-2 cells. The crude extract was obtained with a 50% hydroethanolic extract and then partitioned with hexane, ethyl acetate, and n-butanol. The ethyl-acetate fraction (EAF) showed antiproliferative activity. This fraction was subjected to sequential column chromatography over silica gel to afford onopordopicrin (1), mixtures of 1 with dehydromelitensin-8-(4'-hydroxymethacrylate) (2), a mixture of 2 with dehydromelitensin (3), mixture of 1 with melitensin (4), dehydrovomifoliol (5), and loliolide (6). The compounds were identified by spectroscopic methods (NMR, MS) and comparison with literature data. This is the first description of compounds 2-5 from this species. The compounds tested in Caco-2 cells showed the following CC(50) (µg/mL) values: 1: 19.7 ± 3.4, 1 with 2: 24.6 ± 1.5, 2 with 3: 27 ± 11.7, 1 with 4: 42 ± 13.1, 6 30 ± 6.2; compound 5 showed no activ...Continue Reading

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Citations

Jun 18, 2016·International Journal of Biological Macromolecules·Juliane CarlottoThales R Cipriani
Feb 6, 2019·International Journal of Molecular Sciences·Sang Hee ParkJae Youl Cho
Jul 5, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Masahiko SuzukiHisashi Kato-Noguchi
Jul 6, 2014·Archives of Pharmacal Research·Hyo Hyun YangJae-Ryong Kim
Sep 16, 2017·Experimental and Therapeutic Medicine·Chi-Ting HorngFu-An Chen
Apr 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dong Hee KimMin Jung Kim
Jul 25, 2019·Frontiers in Plant Science·Dongdong WangAtanas G Atanasov
Sep 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pelin TastanJudit Hohmann
Jun 9, 2020·Scientifica·Mohamad Taleb AghaAmin Malik Shah Abdul Majid
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jae Sik YuKi Hyun Kim
Mar 20, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Ibrahim MahmoodShazrul Fazry
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nour El KhatibNathalie Saint
Nov 9, 2021·Journal of Food Biochemistry·Akihiro MaetaKyoko Takahashi

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Datasets Mentioned

BETA
RFA
35777

Methods Mentioned

BETA
NMR

Software Mentioned

GraphPad Prism
GraphPad

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