Evaluation of the Aries Bordetella Assay for Detection and Identification of Bordetella pertussis in Nasopharyngeal Swab Specimens

Journal of Clinical Microbiology
T McMillenN Esther Babady

Abstract

The Aries Bordetella assay (Aries BA) (Luminex Corporation) recently received FDA clearance for the detection and differentiation of Bordetella pertussis and Bordetella parapertussis nucleic acids in nasopharyngeal swab (NPS) samples. The objective of this study was to evaluate the performance of the Aries BA in comparison to that of the BioFire FilmArray respiratory panel (RP). The Aries BA was evaluated using retrospective, remnant nasopharyngeal swabs (NPS), previously tested by FilmArray RP. Performance characteristics evaluated included positive percent agreement (PPA) and negative percent agreement (NPA) with the FilmArray RP. Discordant analysis was performed using bidirectional sequencing. A time and motion study was performed to compare the laboratory workflow of the two tests. Three hundred samples were included in the study. There were no samples positive for B. parapertussis The PPA and NPA of the Aries BA were 61.1% (95% confidence interval [CI], 35.8 to 82.7%) and 100% (95% CI, 98.7 to 100%). Discordant results included five Bordetella bronchiseptica results that were incorrectly identified as B. pertussis by the FilmArray RP and one false-negative result for both the Aries BA and the FilmArray RP. The overall agr...Continue Reading

References

Jul 1, 1991·Clinical Microbiology Reviews·B F Woolfrey, J A Moody
Jun 18, 2017·The Lancet Infectious Diseases·Karene Hoi Ting YeungRaymond Christiaan W Hutubessy
Jun 4, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Moira C McNultyDavid Pitrak

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Citations

Oct 16, 2020·Journal of Clinical Microbiology·Siu-Kei ChowTed E Schutzbank
Jan 29, 2022·Environmental Science and Pollution Research International·Juan CheWei Zhang

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