Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses: Part I--precision, linearity, and accuracy of complete blood cell count

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Natali BauerAndreas Moritz

Abstract

The automated laser-based hematology analyzer Sysmex XT-2000iV™ providing a complete blood cell count (CBC) and 5-part differential has been introduced in large veterinary laboratories. The aim of the current study was to determine precision, linearity, and accuracy of the Sysmex analyzer. Reference method for the accuracy study was the laser-based hematology analyzer ADVIA® 2120. For evaluation of accuracy, consecutive fresh blood samples from healthy and diseased cats (n = 216), dogs (n = 314), and horses (n = 174) were included. A low intra-assay coefficient of variation (CV) of approximately 1% was seen for the CBC except platelet count (PLT). An intra-assay CV ranging between 2% and 5.5% was evident for the differential count except for feline and equine monocytes (7.7%) and horse eosinophils (15.7%). Linearity was excellent for white blood cell count (WBC), hematocrit value, red blood cell count (RBC), and PLT. For all evaluated species, agreement was excellent for WBC and RBC, with Spearman rank correlation coefficients (r(s)) ranging from >0.99 to 0.98. Hematocrit value correlated excellently in cats and dogs, whereas for horses, a good correlation was evident. A good correlation between both analyzers was seen in felin...Continue Reading

References

Jan 1, 1987·American Journal of Clinical Pathology·P B BollingerJ M Trujillo
Jul 15, 2003·American Journal of Veterinary Research·Andreas MoritzDouglas J Weiss
Mar 11, 2005·American Journal of Veterinary Research·Andreas MoritzDouglas J Weiss
Mar 3, 2006·Veterinary Clinical Pathology·Tommaso FurlanelloLaia Solano-Gallego
Sep 13, 2006·Veterinary Clinical Pathology·Asger Lundorff Jensen, Mads Kjelgaard-Hansen
May 4, 2007·International Journal of Laboratory Hematology·C BriggsS J Machin
Apr 28, 2009·Veterinary Clinical Pathology·Inger Lilliehöök, Harold Tvedten
Feb 6, 2010·The Veterinary Journal·S WeissenbacherH Lutz

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Citations

May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yasufumi TakahashiTomokazu Matsue
Apr 28, 2009·Veterinary Clinical Pathology·Inger Lilliehöök, Harold Tvedten
Oct 2, 2009·Veterinary Clinical Pathology·Lise NielsenAnnemarie T Kristensen
Feb 14, 2012·Veterinary Clinical Pathology·Natali B BauerAndreas Moritz
May 4, 2012·Veterinary Clinical Pathology·Iris KampfmannAndreas Moritz
Jun 6, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Alexander KratzGail Kling
Nov 28, 2015·Veterinary Clinical Pathology·Lucy WoolfordJanet M Lanyon
Aug 29, 2018·Veterinary Clinical Pathology·Bente FlatlandRandolph M Baral
Mar 6, 2019·Journal of the American Veterinary Medical Association·Austin K ViallShannon J Hostetter
Jul 30, 2014·Veterinary Clinical Pathology·Alejandro Perez-EcijaFrancisco J Mendoza
Jun 12, 2020·Veterinary Clinical Pathology·Sorawat ThongsahuanKanitta Srinoun
Mar 7, 2021·Animals : an Open Access Journal From MDPI·Mara BagardiClaudio Pigoli
Jun 22, 2021·Veterinary Clinical Pathology·Margot GrebertCatherine Trumel

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Graph Pad Prism Analyse - it Excel
ADVIA

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