Evaluation of the biocontrol efficacy of a Serratia marcescens strain indigenous to tea rhizosphere for the management of root rot disease in tea

PloS One
Gargee Dhar PurkayasthaDipanwita Saha

Abstract

The aim of the present study is to evaluate plant growth promoting and biocontrol efficacy of a Serratia marcescens strain ETR17 isolated from tea rhizosphere for the effective management of root rot disease in tea. Isolated bacterial culture ETR17 showed significant level of in vitro antagonism against nine different foliar and root pathogens of tea. The phenotypic and molecular characterization of ETR17 revealed the identity of the bacterium as Serratia marcescens. The bacterium was found to produce several hydrolytic enzymes like chitinase, protease, lipase, cellulase and plant growth promoting metabolites like IAA and siderophore. Scanning electron microscopic studies on the interaction zone between pathogen and antagonistic bacterial isolate revealed severe deformities in the fungal mycelia. Spectral analyses (LC-ESI-MS, UV-VIS spectrophotometry and HPLC) and TLC indicated the presence of the antibiotics pyrrolnitrin and prodigiosin in the extracellular bacterial culture extracts. Biofilm formation by ETR17 on polystyrene surface was also observed. In vivo application of talc-based formulations prepared with the isolate ETR17 in tea plantlets under green house conditions revealed effective reduction of root-rot disease as ...Continue Reading

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Citations

Sep 6, 2019·Biomolecules·Shraddha PawarDhananjaya P Singh
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·San-Lang WangAnh Dzung Nguyen
Dec 12, 2018·Microbiology Resource Announcements·Søs I DichmannRosanna C Hennessy
Mar 13, 2021·Journal of Biological Engineering·Seong Yeol ChoiRobert J Mitchell
Dec 12, 2021·Journal of Basic Microbiology·Muhammad Saqib MalikMuhammad Anees

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Methods Mentioned

BETA
PCR
ELISA
scanning electron microscopy
column chromatography
gel column chromatography

Software Mentioned

BLAST
SPSS

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