Evaluation of the Cytotoxic and Autophagic Effects of Atorvastatin on MCF-7 Breast Cancer Cells

Balkan Medical Journal
Tuğba Alarcon MartinezSevda Müftüoğlu

Abstract

Recently, cytotoxic effects of statins on breast cancer cells have been reported. However, the mechanism of anti-proliferative effects is currently unknown. Autophagy is non-apoptotic programmed cell death, which is characterized by degradation of cytoplasmic components and as having a role in cancer pathogenesis. To investigate the anti-proliferative effects of atorvastatin on MCF-7 human breast adenocarcinoma cells with respect to both autophagy and apoptosis. Cell culture study. Cell viability was analyzed using WST-1 cell proliferation assay. Apoptosis was determined by the TUNEL method, whereas autophagy was assessed by Beclin-1 and LC3B immunofluorescence staining. Ultrastructural analysis of cells was performed by electron microscopy. Atorvastatin reduced MCF-7 cell proliferation in a dose- and time-dependent manner inducing TUNEL-, Beclin-1-, and LC3B-positive cells. Moreover, ultrastructural analysis showed apoptotic, autophagic, and necrotic morphological changes in treatment groups. A statistically significant increase in the apoptotic index was detected with higher concentrations of atorvastatin at 24 h and 48 h (p<0.05). The anti-proliferative effects of atorvastatin on breast cancer cells is mediated by the induct...Continue Reading

Citations

Jun 19, 2019·Journal of Clinical Medicine·Lídia CedóJoan Carles Escolà-Gil
Aug 20, 2020·Seminars in Cancer Biology·Amir R AfshariAmirhossein Sahebkar
Nov 30, 2020·Seminars in Cancer Biology·Giovanna RevillaJoan Carles Escolà-Gil
Jul 26, 2021·Journal of Experimental & Clinical Cancer Research : CR·Wen JiangXin-Yang He
Nov 14, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Debasish MohapatraShantibhusan Senapati

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