Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines

Oncology Letters
Toktam MemarianiShabnam Shahsavand

Abstract

Cyperus longus is one of the Iranian endemic species. However, to date, and to the best of our knowledge, there are no availale academic reports on the cytotoxicity of this plant. Thus, this study was carried out to examine the in vitro anti-proliferative and anti-apoptotic effects of Cyperus longus extract, fractions and essential oil (EO) on MCF7 and PC3 cell lines. The chemical constituents of EO were identified using gas chromatography (GC)-mass spectrometry (MS) analysis. The cells were cultured in RPMI-1640 medium and incubated with various concentrations of the plant extract and fractions. Cell viability was quantified by MTT assay following 24, 48 and 72 h of exposure to (12.5-200 µg/ml) of the methanol extract, the dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and water fractions, as well as the EO of the plant. The percentage of apoptotic cells was determined using propidium iodide staining of DNA fragments by flow cytometry (sub-G1 peak). The most effective fraction in the MCF7 cell line was the CH2Cl2 fraction (IC50 after 48 h, 25.34±2.01). The EtOAc fraction (IC50 after 48 h, 35.2±2.69) and the methanol extract (IC50 after 48 h, 64.64±1.64) were also found to be effective. The IC50 values obtained for the PC3 cel...Continue Reading

References

Oct 27, 2006·Chemico-biological Interactions·Ramar Perumal SamySavarimuthu Ignacimuthu
Apr 6, 2007·Chemical & Pharmaceutical Bulletin·Jui-Hsin SuJyh-Horng Sheu
Dec 7, 2007·The Journal of Pharmacy and Pharmacology·Jean Legault, André Pichette
Oct 24, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Günter SeelingerChristoph M Schempp
Mar 31, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E Ben-AryeE Lev
Apr 19, 2011·European Journal of Medicinal Chemistry·Vasilis P AndroutsopoulosDemetrios A Spandidos
Jul 12, 2011·Basic & Clinical Pharmacology & Toxicology·Kirsi Myöhänen, Kirsi Vähäkangas
Sep 23, 2011·Pharmaceutical Biology·Seyed Hadi MousaviBizhan Malaekeh-Nikouei
Jan 10, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ping WangDong-hong Yu
Feb 9, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Haisheng ZhangXingbin Yang
Jul 4, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sylwia BorskaPiotr Dziegiel
Sep 18, 2012·Advances in Nutrition·Angela J HendersonElizabeth P Ryan
Feb 6, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Anna ApostolouDimitrios Kouretas
Mar 16, 2013·Phytotherapy Research : PTR·Bizhan Malaekeh-NikoueiSeyed Adel Moallem
Oct 16, 2013·Molecular Cancer Therapeutics·Huanjie ShaoChunrong Yu
May 7, 2014·Oxidative Medicine and Cellular Longevity·You-Qiu XueZhi Shi
Apr 10, 2015·Planta medica·Emmanoel V CostaDaniel P Bezerra
Dec 2, 2015·Oncology Letters·Octavian Tudorel OlaruAristidis M Tsatsakis
Jan 1, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E Ben-AryeE Lev

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Citations

Jun 12, 2017·Neonatology·Roseanne J S VliegenthartAnton H van Kaam
Apr 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elaine C D GonçalvesRafael C Dutra
Apr 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Fiza Ur RehmanSara Qaisar

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Methods Mentioned

BETA
ELISA
flow cytometry

Software Mentioned

FACScan
CellQuest

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