Evaluation of the cytotoxicity and genotoxicity of extracts of mussels originating from Moroccan Atlantic coast, in human colonic epithelial cells Caco-2

Environmental Toxicology
B NasserEdmond E Creppy

Abstract

Industrial processing of phosphates generates chemical wastes which are, without any treatment, discharged directly into the Atlantic Ocean at Jorf Lasfar (JL), located 120 km south of Casablanca (Morocco) were shellfish are also collected by people without any control. Marine bivalves concentrate these pollutants by filtration and serve as vectors in human's exposure. The objective of this study was to test and compare in vitro on human intestinal cells (Caco-2) the cytotoxicity and genotoxicity of mussels (Mytilus galloprovincialis) extracts (either hydrophilic or lipophilic) collected at two coastal sites; JL (neighboring a phosphate processing plat-form) and Oualidia (OL) (a vegetable growing area) located 160 km south of Casablanca (i.e. 40 km south of JL). Using Caco-2 cells, the following end-points have been evaluated, cytotoxicity as measured by MTS test, inhibition of cellular macromolecules syntheses (DNA and protein) and genotoxicity evaluated by DNA fragmentation in agarose gel electrophoresis. The results indicated, that hydrophilic and lipophilic OL mussels extracts are cytotoxic and inhibit cellular macromolecules syntheses. Moreover these extracts damage the DNA in Caco-2 cells. The lipophilic JL mussels extrac...Continue Reading

References

Jun 28, 1996·Journal of Toxicology and Environmental Health·W G MatiasE E Creppy
Mar 4, 2000·Environmental Health Perspectives·F M Van Dolah
Jul 11, 2001·Marine Environmental Research·A Muñoz-BarbosaG Flores-Muñoz
Feb 1, 2002·Toxicon : Official Journal of the International Society on Toxinology·Paulo Vale, Maria Antónia de M Sampayo
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Jul 15, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Véronique MarieAlain Boudou

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