Evaluation of the cytotoxicity and genotoxicity of the spermicides nonoxynol-9 and octoxynol-9

Toxicology Letters
H S ButtarT I Matula


The cytotoxic and genotoxic potentials of the spermicidal agents, nonoxynol-9 (N-9) and octoxynol-9 (0-9), were evaluated in in vitro test systems using rat liver cells. N-9 was also tested for the induction of sperm abnormalities in mice. Dose-related cytocidal effects were seen after the addition of N-9 and O-9 to the culture medium for 24 h. The mean concentrations of N-9 and O-9 necessary to decrease the number of viable cells by 50% (LC50) were 24 and 43 micrograms/ml of media, respectively. The spermicides neither induced DNA repair in freshly isolated hepatocytes, nor caused any mutations at HGPRT locus in the T51B rat liver cell line. There was also a lack of malignant transformation response in the low-calcium assay. Further, the germinal cells of mice remained unaffected by N-9.


Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S H SwierengaS Karasaki
Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Mar 1, 1985·Fertility and Sterility·J L MillsH W Berendes
Oct 1, 1969·Journal of Reproduction and Fertility·P EcksteinA J Sobrero
May 7, 1982·JAMA : the Journal of the American Medical Association·S ShapiroS P Helmrich
Sep 1, 1982·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·L Tryphonas, H S Buttar
Apr 1, 1982·American Journal of Public Health·K J Rothman
Nov 5, 1982·JAMA : the Journal of the American Medical Association·J L MillsH W Berendes
Aug 1, 1982·Teratology·A P PolednakD M Glebatis
Mar 13, 1980·The New England Journal of Medicine·J E Gottesman

Related Concepts

DNA, Double-Stranded
Base Excision Repair
Injections, Intraperitoneal
Lethal Dose 50
Mutagenicity Tests
Y-Chromosome-Bearing Sperm

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