Evaluation of the cytotoxicity of 10 chemicals in human and rat hepatocytes and in cell lines: Correlation between in vitro data and human lethal concentration

Toxicology in Vitro : an International Journal Published in Association with BIBRA
X PonsodaM J Gómez-Lechón

Abstract

The cytotoxicity of 10 chemicals from the Multicentre Evaluation of In vitro Cytotoxicity (MEIC) list (nos 21-30) was evaluated in human and rat cultured hepatocytes and in two established cell lines (HepG2 and 3T3) according to the MEIC programme organized by the Scandinavian Society of Cell Toxicology. The MTT test was used as the endpoint of cytotoxicity after 24hr of exposure to the chemicals. Theophylline, phenobarbital and paraquat were the least cytotoxic compounds in the cellular systems (IC(50) = 450-17,000 mum) except for the 3T3 cells. The seven remaining chemicals (dextropropoxyphene, propranolol, arsenic trioxide, cupric sulfate, mercuric chloride, thioridazine and thallium sulfate) showed a similar relative cytotoxic ranking in the four in vitro systems in the lower range of concentrations (IC(50) = 2-350 mum). The data suggest that these 10 chemicals have a basal cytotoxic effect common to the four in vitro systems, and probably none of these compounds could be considered either hepatotoxic or species specific. The correlation between in vitro data and human lethal blood concentrations showed that the predictability of the in vitro systems was similar to that of in vivo rodent tests (LD(50)) only when low cytotox...Continue Reading

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Citations

May 17, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F LestariB Markovic
May 17, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Argelia Castaño, María José Gómez-Lechón
Jun 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C J WaterfieldJ A Timbrell
Sep 1, 2007·Journal of Toxicology and Environmental Health. Part a·Shahnaz BakandChris Winder
Dec 24, 2013·Life Sciences·Mengyuan ZhengMuralikrishnan Dhanasekaran
May 20, 2005·Cell and Tissue Research·Amagoia Gómez-MendikuteMiren P Cajaraville
Jun 19, 2004·Inhalation Toxicology·Chrysanthus J ObotAndrij Holian
Nov 1, 2015·Ecotoxicology and Environmental Safety·Pavel HrouzekJiří Kopecký
Sep 14, 2012·Drug and Chemical Toxicology·Juan José Rodríguez-Mercado, Mario Agustín Altamirano-Lozano
Aug 5, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ludovic PeyreRoger Rahmani
Jul 15, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R HamidP Bullock
Jun 22, 2018·Clinical and Experimental Nephrology·Bart DioosAnders P Wieslander
May 15, 2018·Frontiers in Public Health·Steven D ForsytheAleksander Skardal
Jan 1, 2000·Alternatives to Laboratory Animals : ATLA·S KorkmazH Can Baser
Jan 1, 2020·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Muhammad Hidhir KhaworyHabibah A Wahab
Jun 22, 2021·Journal of Pharmacological and Toxicological Methods·Adela KrivohlavekAna-Marija Domijan

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