Evaluation of the cytotoxicity of organic dust components on THP1 monocytes-derived macrophages using high content analysis

Environmental Toxicology
Eve Ramery, Peter J O'Brien

Abstract

Organic dust contains pathogen-associated molecular patterns (PAMPs) which can induce significant airway diseases following chronic exposure. Mononuclear phagocytes are key protecting cells of the respiratory tract. Several studies have investigated the effects of PAMPs and mainly endotoxins, on cytokine production. However the sublethal cytotoxicity of organic dust components on macrophages has not been tested yet. The novel technology of high content analysis (HCA) is already used to assess subclinical drug-induced toxicity. It combines the capabilities of flow cytometry, intracellular fluorescence probes, and image analysis and enables rapid multiple analyses in large numbers of samples. In this study, HCA was used to investigate the cytotoxicity of the three major PAMPs contained in organic dust, i.e., endotoxin (LPS), peptidoglycan (PGN) and β-glucans (zymosan) on THP-1 monocyte-derived macrophages. LPS was used at concentrations of 0.005, 0.01, 0.02, 0.05, 0.1, and 1 μg/mL; PGN and zymosan were used at concentrations of 1, 5, 10, 50, 100, and 500 μg/mL. Cells were exposed to PAMPs for 24 h. In addition, the oxidative burst and the phagocytic capabilities of the cells were tested. An overlap between PGN intrinsic fluoresce...Continue Reading

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Citations

Sep 26, 2015·International Journal of Environmental Research and Public Health·Lili WangHuiming Chen
Apr 15, 2016·Journal of Immunotoxicology·Lucia E RosasTracey L Papenfuss
Jun 6, 2016·Allergology International : Official Journal of the Japanese Society of Allergology·Masanari WatanabeEiji Shimizu
Sep 17, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Shenxuan LiangXiaozhong Yu
Sep 17, 2018·Journal of Nanobiotechnology·Ashraf AbdelkhaliqHans Bouwmeester

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