Evaluation of the dark-medium objective lens in counting asbestos fibers by phase-contrast microscopy

The Annals of Occupational Hygiene
Eun Gyung LeeMartin Harper

Abstract

A Japanese round-robin study revealed that analysts who used a dark-medium (DM) objective lens reported higher fiber counts from American Industrial Hygiene Association (AIHA) Proficiency Analytical Testing (PAT) chrysotile samples than those using a standard objective lens, but the cause of this difference was not investigated at that time. The purpose of this study is to determine any major source of this difference by performing two sets of round-robin studies. For the first round-robin study, 15 AIHA PAT samples (five each of chrysotile and amosite generated by water-suspended method, and five chrysotile generated by aerosolization method) were prepared with relocatable cover slips and examined by nine laboratories. A second round-robin study was then performed with six chrysotile field sample slides by six out of nine laboratories who participated in the first round-robin study. In addition, two phase-shift test slides to check analysts' visibility and an eight-form diatom test plate to compare resolution between the two objectives were examined. For the AIHA PAT chrysotile reference slides, use of the DM objective resulted in consistently higher fiber counts (1.45 times for all data) than the standard objective (P-value <...Continue Reading

References

Sep 1, 1970·American Industrial Hygiene Association Journal·J R LynchD L Johnson
Apr 12, 2003·AIHA Journal : a Journal for the Science of Occupational and Environmental Health and Safety·Martin Harper, Al Bartolucci
Jan 5, 2008·Journal of Environmental Monitoring : JEM·Thomas W S Pang, Martin Harper
Feb 13, 2009·Journal of Environmental Monitoring : JEM·Martin HarperThomas W S Pang
Aug 1, 1947·Journal of the Optical Society of America·A T BRICE, P H KECK
Jun 7, 2011·The Annals of Occupational Hygiene·Eun Gyung LeeMartin Harper

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Citations

Sep 28, 2016·The Annals of Occupational Hygiene·Tomoki NishimuraAkio Kuroda

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