Evaluation of the Digene Hybrid Capture System for detection and quantitation of human cytomegalovirus viremia in human immunodeficiency virus-infected patients.

Journal of Clinical Microbiology
T MazzulliS Walmsley

Abstract

The Digene Hybrid Capture System (DHCS) is a solution hybridization antibody capture assay for the chemiluminescent detection and quantitation of cytomegalovirus (CMV) DNA in leukocytes. This assay was compared with the CMV antigenemia assay and shell vial and tube cultures for the detection of CMV in 234 blood specimens from 72 patients with human immunodeficiency virus. Intra- and interrun precision of the DHCS assay gave coefficients of variation of 17.8 and 16.3%, respectively. The correlation coefficient for the quantitative results obtained by the DHCS assay and the antigenemia assay was 0.911 (95% confidence interval, 0.885 to 0.930). Agreement between the DHCS assay and the other three assays ranged from 83 to 86%. The DHCS assay detected 71, 87, and 84% of specimens that were positive by antigenemia, shell vial cultures, and tube culture, respectively. A total of 92% of specimens that were positive by the DHCS assay were also positive by at least one of the other assays. Evaluation of the usefulness of quantitation of CMV DNA by using the DHCS assay and its correlation with clinical disease demonstrated that, with some exceptions, patients with clinical CMV disease tended to have high levels of DNA whereas asymptomatic...Continue Reading

References

Feb 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W L Drew
Jul 1, 1984·The Journal of Infectious Diseases·S A SpectorR McMillan
Jun 1, 1994·Journal of Clinical Microbiology·H D Engler, S T Selepak
Oct 1, 1995·Journal of Clinical Microbiology·D ZipetoL Rasmussen

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Citations

Nov 14, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P RautenbergD Franke
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·R Abu-Nader, R Patel
Oct 5, 2002·Clinical Microbiology Reviews·Maria Grazia Revello, Giuseppe Gerna
Oct 5, 2013·Clinical Microbiology Reviews·Raymund R Razonable, Randall T Hayden
Oct 6, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G A Storch
Jul 1, 1998·Clinical and Diagnostic Virology·R L Hodinka
Dec 19, 2000·International Journal of Antimicrobial Agents·C C Ginocchio
Oct 4, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Rafael E de la HozChristopher Sherlock
Jul 17, 1998·Clinical Microbiology Reviews·M Boeckh, G Boivin
Aug 15, 1998·Journal of Clinical Microbiology·C Y TongA Bakran
Feb 3, 2000·Journal of Clinical Microbiology·P WattanamanoM A Beilke

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