Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma

Clinica Chimica Acta; International Journal of Clinical Chemistry
Christian BundschuhBenjamin Dieplinger

Abstract

Besides SARS-CoV-2 RT-PCR testing, serological testing is emerging as additional option in COVID-19 diagnostics. Aim of this study was to evaluate novel immunoassays for detection of SARS-CoV-2 antibodies in human plasma. Using EDITM Novel Coronavirus COVID-19 Enzyme Linked Immunosorbent Assays (ELISAs), we measured SARS-CoV-2 IgM and IgG antibodies in 64 SARS-CoV-2 RT-PCR confirmed COVID-19 patients with serial blood samples (n = 104) collected at different time points from symptom onset. Blood samples from 200 healthy blood donors and 256 intensive care unit (ICU) patients collected before the COVID-19 outbreak were also used. The positivity rates in the COVID-19 patients were 5.9% for IgM and 2.9% for IgG ≤ 5 days after symptom onset; Between day 5 and day 10 the positivity rates were 37.1% for IgM and 37.1% for IgG and rose to 76.4% for IgM and 82.4% for IgG after > 10-15 days. After 15-22 days the "true" positivity rates were 94.4% for IgM and 100% for IgG. The "false" positivity rates were 0.5% for IgM and 1.0% for IgG in the healthy blood donors, 1.6% for IgM and 1.2% for IgG in ICU patients. This study shows high "true" vs. low "false" positivity rates for the EDITM SARS-CoV-2 IgM and IgG ELISAs.

Citations

Jan 5, 2021·Applied Microbiology and Biotechnology·Praveen RaiIddya Karunasagar
Nov 1, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hadi M YassineGheyath K Nasrallah
Jan 19, 2021·Proceedings·Jonathan KopelAbhilash Perisetti
Mar 26, 2021·Infection and Drug Resistance·Mumtarin Jannat OisheeNihad Adnan
Sep 1, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Susmita ChaudhuriUNKNOWN DBT India Consortium for COVID-19 Research
May 1, 2021·Diagnostic Microbiology and Infectious Disease·Mario HönemannUwe Gerd Liebert
Jun 27, 2021·Bulletin of Experimental Biology and Medicine·A V AleshkinA V Karaulov
Oct 23, 2020·Recent Patents on Nanotechnology·P ArandaE Ruiz-Hitzky

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Methods Mentioned

BETA
Enzyme Linked Immunosorbent Assay
ELISAs
ELISA

Software Mentioned

SPSS
MedCalc

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