Evaluation of the effect of miltefosine on Trichomonas vaginalis

Parasitology Research
Débora Afonso Silva RochaMarlene Benchimol

Abstract

Trichomonas vaginalis causes trichomoniasis in humans, a sexually transmitted disease commonly treated with metronidazole (MTZ). MTZ is known to cause undesirable side effects, and MTZ-resistant parasites have been reported. Thus, the development of an alternative treatment is desirable. Miltefosine (MLT) is an alkylphosphocholine synthetic lipid analogue that displays antiparasitic activity against Leishmania, Trypanosoma cruzi, Entamoeba histolytica, Acanthamoeba spp., Giardia lamblia, T. vaginalis and some fungi. Moreover, it has been used for oral treatment of visceral leishmaniosis in several countries. Here, we analysed the MLT-induced antiproliferative effect on T. vaginalis as well its effect on the fine structure and viability of the parasite. We observed a dose-dependent effect with an IC50 of 14.5 and 20 μM after 24 and 48 h, respectively. Furthermore, reversibility assays demonstrated that new incubations were necessary in order to maintain the antiproliferative effect. Ultrastructural analyses demonstrated that MLT induced several alterations, including the appearance of wrinkled and rounded cells, membrane blebbing, intense vacuolization and nuclear condensation, all indicative of cell death by apoptosis. In addit...Continue Reading

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Citations

Jun 2, 2014·Parasitology Research·Adrine Maria InnocenteTiana Tasca
Jan 8, 2015·Parasitology Research·Zhiyue LvHao Li
May 3, 2019·Parasitology·Victor MidlejMarlene Benchimol
May 30, 2020·Ocular Immunology and Inflammation·Andrea NaranjoGuillermo Amescua
Oct 28, 2019·American Journal of Ophthalmology Case Reports·Kristin E HirabayashiChristopher N Ta
Mar 3, 2018·Experimental Parasitology·Viviane Sant'AnnaWanderley de Souza

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