Evaluation of the effects of animal age, concurrent bacterial infection, and pathogenicity of porcine reproductive and respiratory syndrome virus on virus concentration in pigs

American Journal of Veterinary Research
Jenny G ChoHan Soo Joo

Abstract

To evaluate the influences of animal age, bacterial coinfection, and porcine reproductive and respiratory syndrome virus (PRRSV) isolate pathogenicity on virus concentration in pigs. Twenty-one 2-month-old pigs and eighteen 6-month-old pigs. Pigs were grouped according to age and infected with mildly virulent or virulent isolates of PRRSV. The role of concurrent bacterial infection was assessed by infecting selected pigs with Mycoplasma hyopneumoniae 21 days prior to inoculation with PRRSV. On alternating days, blood and swab specimens of nasal secretions and oropharyngeal secretions were collected. On day 21 after inoculation with PRRSV, selected tissues were harvested. Concentrations of PRRSV were determined by use of quantitative real-time PCR and expressed in units of TCID(50) per milliliter (sera and swab specimens) or TCID(50) per gram (tissue specimens). Concentrations of virus were higher in blood and tonsils of pigs infected with virulent PRRSV. Pigs infected with virulent PRRSV and M hyopneumoniae had significantly higher concentrations of viral RNA in lymphoid and tonsillar tissue. Coinfection with M hyopneumoniae resulted in a higher viral load in oropharyngeal swab specimens and blood samples, independent of virule...Continue Reading

References

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Citations

Aug 31, 2010·Virus Research·Michael P MurtaughFrederick C-C Leung
May 30, 2006·Theriogenology·Jenny G Cho, Scott A Dee
Jul 11, 2009·Microbiology·Cláudio X MachadoHenrique B Ferreira
Jun 1, 2012·Veterinary Immunology and Immunopathology·Gabriela Calzada-NovaFederico A Zuckermann
Apr 2, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carmen AlonsoMontserrat Torremorell
Sep 8, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Albert RoviraClaudia Muñoz-Zanzi
Sep 8, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Albert RoviraClaudia Muñoz-Zanzi
Dec 14, 2011·Animal Health Research Reviews·T OpriessnigP G Halbur
Jan 18, 2011·Transboundary and Emerging Diseases·Y S MalikS Chakravarti

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