Oct 16, 1989

Evaluation of the effects of erythro-9(2-hydroxy-3-nonyl) adenine (EHNA) on HIV-1 production in vitro

Biochemical and Biophysical Research Communications
Y SeiP K Arora

Abstract

Effects of erythro-9(2-hydroxy-3-nonyl) adenine (EHNA), an inhibitor of the common Adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4.), on HIV-1 production was evaluated in vitro. Reverse transcriptase (RT) activity in the supernatant was inhibited by nearly 50% in EHNA-treated HIV-1 infected H9 cells, when compared with untreated but infected H9 cells. There was also a significant decrease in cell viability, but this was reversed following the addition of deoxycytidine (dC) to these cultures. The combined treatment was also effective in suppressing HIV-1 release from HIV-1-infected U937 cells. This combined EHNA plus dC treatment had no effect on RT activity in the cell lysates, suggesting that the inhibition of HIV-1 production may be due to the disturbance of virus release from infected cells.

  • References5
  • Citations2

References

Mentioned in this Paper

Flow Cytometry
Virus Replication
9-(2-hydroxy-3-nonyl)adenine, (S-(R*, S*))-isomer
Adenosine Deaminase Inhibitors
Drug Augmentation
ADA
RNA-Directed DNA Polymerase
HIV-1
Deoxycytidine
HIV Envelope Protein gp120

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