PMID: 8972722Nov 8, 1996Paper

Evaluation of the effects of immunosuppressants on neuronal and glial cells in vitro by multinuclear magnetic resonance spectroscopy

Biochimica Et Biophysica Acta
Natalie SerkovaD Leibfritz

Abstract

The use of the undecapeptide cyclosporine and the macrolide tacrolimus as immunosuppressants in transplantation medicine and for the therapy of immune diseases often provokes side effects, among the most important one is neurotoxicity. Changes in the cellular metabolism of glial cells (C6 rat glioma), neuronal cells (N1E-115 mouse neuroblastoma) and primary glia cells (isolated from rats) after addition of cyclosporine and tacrolimus were investigated using 1H-, 13C- and 31P-NMR spectroscopy in vitro. Cells were exposed to various concentrations of the drugs from 3 h to 42 days. The immunosuppressants (cyclosporine IC50 : 55 mumol/l; tacrolimus IC50 : 47 mumol/l) inhibited cell proliferation in a concentration- and time-dependent fashion. Multinuclear NMR studies of PCA extracts of drug-treated cells showed a significant deterioration in the energy status (a decreasing level of PCr : -46 +/- 11%; an increasing NDP/NTP ratio: +136 +/- 4% and an increasing level of Pi : +248 +/- 15%; mean +/- standard deviation). It also showed decreasing concentrations of major cell metabolites like NAA (-59 +/- 12%) in neuroblastoma cells and myo-inositol (-47 +/- 6%) in glia cells compared with untreated controls. Immunosuppressive treatment c...Continue Reading

References

Aug 1, 1988·Journal of Neuroscience Research·H Pasantes Morales, A Schousboe
Jul 26, 1968·Science·P BendaW Sweet
Dec 16, 1994·Biochemical Pharmacology·N Kraus-Friedmann, L Feng

❮ Previous
Next ❯

Citations

Oct 13, 2012·Journal of Proteome Research·Jelena KlawitterUwe Christians
Jun 18, 2011·American Journal of Respiratory and Critical Care Medicine·Natalie J SerkovaKathleen A Stringer
Mar 9, 2010·Breast Cancer Research : BCR·Jelena KlawitterJost Klawitter
Aug 13, 2005·Journal of Ethnopharmacology·Feng Qin, Hong-Xiang Sun
Jun 27, 2014·Immunopharmacology and Immunotoxicology·Shuang GuanJing Lu
Sep 11, 2007·Progress in Neuro-psychopharmacology & Biological Psychiatry·Tayfun I Uzbay
Mar 14, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Natalie SerkovaUwe Christians
Mar 29, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing LuGuanghong Xie
Nov 23, 2005·Anesthesia and Analgesia·Claus U NiemannNatalie Serkova
Jan 1, 2008·Toxicology Mechanisms and Methods·Natalie J SerkovaMartha C Tissot van Patot
Aug 26, 2006·JPEN. Journal of Parenteral and Enteral Nutrition·Zhi Yong PengPaul E Wischmeyer
Aug 17, 2002·The Journal of Pharmacology and Experimental Therapeutics·Claus U NiemannNatalie Serkova
Jun 22, 2000·NMR in Biomedicine·V GovindarajuA A Maudsley
Oct 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sven GottschalkNatalie J Serkova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.