PMID: 8594530Nov 1, 1995Paper

Evaluation of the effects of saline versus bicarbonate-containing mixed salts solutions on rabbit corneal epithelium in vitro

Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)
M J Doughty

Abstract

Rabbit corneas were incubated, over 4 h in vitro, with the corneal epithelial surface exposed to various solutions to assess their utility as incubation solutions for physiological, pharmaceutical or toxicological studies. The corneal endothelium was perfused with a 35 mM bicarbonate-mixed salts solution equilibrated at 36 degrees C. Corneal thickness, corneal hydration or epithelial cell appearance (as assessed by scanning electron microscopy) were found to be similar to in vivo if a 35 mM bicarbonate, mixed salts solution (equilibrated with 5% CO2-air) was used for the epithelium. Some swelling (14 microns h-1), increased hydration and minor cell exfoliation were seen if this 35 mM bicarbonate solution was equilibrated with 5% CO2-95% O2 (hyperoxia). Solutions with only 5 mM bicarbonate (0.5% CO2-air) produced rapid swelling, large increases in hydration and marked cellular damage. Slightly hypertonic (310 mOsm kg-1) solutions containing 5 mM bicarbonate caused some swelling at 15 microns h-1, small increases in hydration and some cell damage but the swelling and cellular damage were further reduced by making the solution slightly more hypertonic (325 mOsm kg-1) by addition of NaCl and KCl. Saline (NaCl 0.9% or 0.97%) or phos...Continue Reading

Citations

Jun 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M J Doughty
Dec 18, 2013·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Kalika L BandamwarQian Garrett
Apr 29, 2005·Micron : the International Research and Review Journal for Microscopy·Michael J Doughty, Jan P G Bergmanson
Nov 3, 2012·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·M J Doughty, S Hagan
May 7, 2010·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Michael J Doughty
Mar 26, 2004·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Michael J Doughty, Jan P G Bergmanson

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