Evaluation of the effects of sugarcane processing on the presence of GM DNA and protein in sugar.

GM Crops & Food
Maria L SerenoChristopher Cullis

Abstract

The Brazilian Sucro-energy Sector produces both energy, in the form of ethanol fuel, industrial steam and electricity, and sugar. Centro de Tecnologia Canavieira (CTC), the leading Brazilian sugarcane breeding company, has developed a pipeline of insect-protected sugarcane varieties to control sugarcane borer damage. The goal of this manuscript is to present the results of studies with three genetically modified (GM) sugarcane varieties and to evaluate the published literature regarding the possible presence of GM sugarcane DNA or protein in raw or refined sugar. Specifically, two varieties of approved GM sugarcane, CTC91087-6 and CTC175-A, and an experimental CTC variety, were grown in four individual plots to produce four batches each of processed raw sugar using standard smaller-scale laboratory processing methods resulting in a total of 12 independent batches of raw sugar. Herein, we report the development of event-specific probes and DNA detection methods, designed to detect the junction of sugarcane genomic DNA and the inserted DNA of the two approved GM varieties. An identical approach was used for the testing of sugar made from the experimental CTC variety. The methodology used TaqMan® real-time PCR and ELISA assays val...Continue Reading

References

Jan 1, 1987·Journal of Molecular Evolution·P M Sharp, W H Li
Nov 9, 2000·Regulatory Toxicology and Pharmacology : RTP·F S BetzR L Fuchs
Mar 28, 2009·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Taichi OguchiKazumi Kitta
Dec 21, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B G Hammond, J M Jez
Oct 30, 2013·Critical Reviews in Toxicology·Bruce HammondUNKNOWN ILSI International Food Biotechnology Committee Task Force on Use of Mammalian Toxicology Studies in Safety Assessment of GM
Apr 11, 2018·Frontiers in Bioengineering and Biotechnology·Adriana Cheavegatti-GianottoWladecir Salles de Oliveira

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Methods Mentioned

BETA
PCR
ELISA
transgenic
Assay
Protein Assay

Software Mentioned

Excel
Softmax Pro
ICUMSA

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