Evaluation of the efficiency of human immune system reconstitution in NSG mice and NSG mice containing a human HLA.A2 transgene using hematopoietic stem cells purified from different sources

Journal of Immunological Methods
John PattonTim Manser

Abstract

Severely immunodeficient mice such as the NOD/SCID/IL2rγ(null) (NSG) strain can be engrafted with human hematopoietic stem cells (HSCs), resulting in chimeric mice containing many components of the human immune system (Human Immune System mice or HIS mice). HIS mice can both support the replication of and recapitulate much of the immunological response to a variety of pathogens, including ones with strict human tropism, such as HIV-1. In an effort to develop a better mouse model for human infectious pathogen infection and possible immune resolution, we compared the human immune system reconstitution of NSG mice following injection with human CD34(+) HSCs purified from either fetal liver (FL) or umbilical cord blood (UCB). We analyzed reconstitution in standard NSG mice as well as a derivative of these mice containing an HLA.A2 encoding transgene (NSG.A2). HSCs from both sources effectively reconstituted hematopoietic lineages when injected into NSG mice. In marked contrast, total CD45(+) human hematopoietic cells in NSG.A2 mice were well reconstituted by HSCs from UCB but very poorly by HSCs purified from FL. Moreover, the reconstitution of T cell lineages in NSG.A2 mice by HSCs from UCB was inferior to that obtained using NSG ...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R R Hardy, K Hayakawa
Jun 1, 1985·The Journal of Experimental Medicine·K HayakawaL A Herzenberg
Jul 2, 2003·Nature Medicine·Melissa Pope, Ashley T Haase
Aug 2, 2005·Nature Reviews. Immunology·Reina E Mebius, Georg Kraal
Oct 24, 2006·Nature Medicine·Michael W MelkusJ Victor Garcia
Jan 30, 2007·Nature Reviews. Immunology·Leonard D ShultzDale L Greiner
Feb 24, 2007·Nature Reviews. Immunology·Kenneth Dorshkind, Encarnacion Montecino-Rodriguez
Nov 6, 2007·Nature Immunology·Katsuto TakenakaJayne S Danska
Jun 2, 2009·Current Opinion in Immunology·Tri Giang PhanJason G Cyster
Jun 3, 2009·The Journal of Experimental Medicine·Till StrowigChristian Münz
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Qingfeng ChenJianzhu Chen
Jun 24, 2011·Trends in Immunology·Tim WillingerRichard A Flavell
Jul 21, 2012·Science Translational Medicine·Timothy E DudekTodd M Allen
Sep 8, 2012·PloS One·Matthew B GreenblattAntonios O Aliprantis
Oct 13, 2012·Nature Reviews. Immunology·Leonard D ShultzDale L Greiner
Jan 3, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert Friedrich KudernatschCarmen Scheibenbogen
Jan 22, 2013·Annual Review of Immunology·Anthony RongvauxMarkus G Manz
Mar 13, 2014·Journal of Immunological Methods·Michael A BrehmLeonard D Shultz

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Citations

Jul 19, 2016·Midwifery·Anna Ledenfors, Carina Berterö
Aug 30, 2016·International Journal of Nursing Knowledge·Cherrill StockmannCaroline Ehlers
Feb 8, 2018·Archivum Immunologiae Et Therapiae Experimentalis·Kylie Su Mei YongQingfeng Chen
Mar 20, 2019·European Journal of Immunology·Guillemette Masse-RansonJames P Di Santo
Apr 11, 2020·Molecular Carcinogenesis·J Jason MortonAntonio Jimeno
Sep 15, 2018·Frontiers in Immunology·Grégory EhxFrédéric Baron
Mar 19, 2020·Frontiers in Immunology·Yoshiki Akatsuka
Feb 16, 2019·Frontiers in Immunology·Frida ArreyStefan H E Kaufmann
Nov 3, 2016·Cancer Research·J Jason MortonAntonio Jimeno
Oct 23, 2020·Molecular Cancer Research : MCR·J Jason MortonAntonio Jimeno

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