Evaluation of the elastic Young's modulus and cytotoxicity variations in fibroblasts exposed to carbon-based nanomaterials

Journal of Nanobiotechnology
Homero F PastranaAlba Ávila

Abstract

The conventional approaches to assess the potential cytotoxic effects of nanomaterials (NMs) mainly rely on in vitro biochemical assays. These assays are strongly dependent on the properties of the nanomaterials, for example; specific surface area (SSA), size, surface defects, and surface charge, and the host response. The NMs properties can also interfere with the reagents of the biochemical and optical assays leading to skewed interpretations and ambiguous results related to the NMs toxicity. Here, we proposed a structured approach for cytotoxicity assessment complemented with cells' mechanical responses represented as the variations of elastic Young's modulus in conjunction with conventional biochemical tests. Monitoring the mechanical properties responses at various times allowed understanding the effects of NMs to the filamentous actin cytoskeleton. The elastic Young's modulus was estimated from the force volume maps using an atomic force microscope (AFM). Our results show a significant decrease on Young's modulus, ~ 20%, in cells exposed to low concentrations of graphene flakes (GF), ~ 10% decrease for cells exposed to low concentrations of multiwalled carbon nanotubes (MWCNTs) than the control cells. These considerable c...Continue Reading

References

Mar 14, 2001·American Journal of Physiology. Cell Physiology·H Lum, K A Roebuck
Apr 20, 2002·Biophysical Journal·Emilios K DimitriadisRichard S Chadwick
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Campbell W Gourlay, Kathryn R Ayscough
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Jun 15, 2006·Nano Letters·J M Wörle-KnirschH F Krug
Mar 27, 2007·Injury·Robert BielbyDennis McGonagle
Jan 31, 2008·Pflügers Archiv : European journal of physiology·Jan Christian Martens, Manfred Radmacher
Sep 9, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Alexandra KrollJürgen Schnekenburger
Jan 12, 2010·Journal of Internal Medicine·G Oberdörster
Jan 30, 2010·Nature·Daniel A Fletcher, R Dyche Mullins
Mar 25, 2010·The Biochemical Journal·Markus KarlssonChristina I Frennesson
Apr 20, 2010·Expert Review of Cardiovascular Therapy·Bethan FreestoneGregory Y H Lip
Jul 16, 2010·Risk Analysis : an Official Publication of the Society for Risk Analysis·Linda C Abbott, Andrew D Maynard
Jul 31, 2010·ACS Nano·Brian D HoltKris Noel Dahl
Aug 24, 2010·Particle and Fibre Toxicology·Syed K SohaebuddinLiping Tang
Feb 25, 2011·Particle and Fibre Toxicology·Alexandra KrollJürgen Schnekenburger
May 26, 2011·Neuron·Daisuke NakadaSean J Morrison
Jul 29, 2011·Nanomedicine·Cordula HirschPeter Wick
Oct 1, 2011·Chemical Research in Toxicology·Vanesa C SanchezAgnes B Kane
Apr 20, 2012·Toxicology and Applied Pharmacology·Anna A ShvedovaValerian E Kagan
Jul 10, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Rachael M CristScott E McNeil
Apr 13, 2013·Angewandte Chemie·Alberto Bianco
May 4, 2013·Methods : a Companion to Methods in Enzymology·Igor SokolovNataliia V Guz
Aug 30, 2013·Journal of the Royal Society, Interface·Ramesh SubbiahKyoung Jin Choi
Sep 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Doris Illner, Harry Scherthan
Jan 15, 2014·The Journal of Physical Chemistry. B·Christopher C ZimmerGang-yu Liu
Feb 1, 2014·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·P A SchulteK F Martinez
Apr 1, 2014·Advances in Experimental Medicine and Biology·Isaac M AdjeiVinod Labhasetwar
Apr 5, 2014·Journal of Internal Medicine·R J BurgessS J Morrison
Apr 15, 2014·Particle and Fibre Toxicology·Hedwig M BraakhuisFlemming R Cassee
Apr 17, 2014·Journal of Biomedical Nanotechnology·Ida Dulińska-MolakGuoping Chen
May 7, 2015·Risk Analysis : an Official Publication of the Society for Risk Analysis·Junko NakanishiSosuke Hanai
Jun 5, 2015·Scientific Reports·Zonglin GuRuhong Zhou
Jun 30, 2015·Scientific Reports·Alexander X Cartagena-RiveraArvind Raman
Jul 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chenbo DongCerasela Zoica Dinu
Aug 6, 2015·Nanomedicine·Fernanda M P TonelliRodrigo R Resende

❮ Previous
Next ❯

Citations

Apr 3, 2020·Archives of Toxicology·Feng XiaoliShao Longquan
Aug 21, 2019·Archives of Toxicology·Gautam MahajanChandrasekhar Kothapalli
Nov 26, 2020·BMC Biology·Daniela Barrios, Laura Segatori
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anja SadžakSuzana Šegota
Oct 27, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinyue GuoJilin Tang

❮ Previous
Next ❯

Methods Mentioned

BETA
AFM

Software Mentioned

ImageJ
OriginPro

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.