Evaluation of the ELISA and comparison to the complement fixation test and radial immunodiffusion enzyme assay for detection of antibodies against Mycoplasma hyopneumoniae in swine serum

Veterinary Microbiology
M BereiterR F Ross

Abstract

An enzyme-linked immunosorbent assay (ELISA) was evaluated for detection of antibodies (Ab) against Mycoplasma hyopneumoniae and M. flocculare in sera from swine experimentally infected with these agents. In addition, the ELISA was compared with the complement fixation test (CFT), and radial immunodiffusion enzyme assay (RIDEA) for the demonstration of Ab against M. hyopneumoniae. Twenty two 6-week-old swine from a respiratory disease-free herd were divided into five groups. Two or three pigs from each of the four groups were inoculated, respectively, with M. hyopneumoniae or with M. flocculare while two pigs in each group were contact exposed to the inoculated penmates. A fifth group, consisting of three pigs, served as inoculated controls. Pigs inoculated with M. hyopneumoniae began coughing 13 days post inoculation (PI). Antibodies were first detected 2 weeks PI with the CFT, 3 weeks PI with the ELISA, and 5 weeks PI with the RIDEA. With the ELISA and RIDEA, Ab were still detectable one year PI at a very low level. With the CFT, Ab were not detectable in sera from any swine beyond 5 months PI. At necropsy 1 year PI, no lesions were detected in lungs of any of the animals nor were mycoplasmas detected. M. flocculare inoculate...Continue Reading

Citations

Aug 13, 2009·Applied Biochemistry and Biotechnology·Yuan-Zuo LiDavid Shiuan
Jan 20, 2011·The Journal of Biological Chemistry·Lisa M SeymourMark J Walker
Oct 19, 2004·Journal of Bacteriology·F Chris MinionGregory G Mahairas
Jun 28, 2001·Journal of Clinical Microbiology·E L ThackerB H Janke
Jun 30, 2005·Animal Health Research Reviews·Eileen L Thacker
Apr 21, 2009·Clinics in Chest Medicine·L Joseph Wheat
Aug 17, 2016·Veterinary Microbiology·Andrew C PetersenF Chris Minion
Sep 15, 2009·Protein Expression and Purification·Simone SimionattoOdir A Dellagostin
Apr 19, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mehrdad AmeriWalter H Hsu
Feb 5, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mehrdad Ameri-MahabadiWalter H Hsu
Mar 2, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Eduardo FanoJohn Deen
Jun 3, 1999·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M CalsamigliaA Trigo
Sep 13, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·K LevonenP Veijalainen
Jan 30, 1999·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M NiangM L Kaeberle
Mar 15, 2018·Frontiers in Cellular and Infection Microbiology·Benjamin B A RaymondSteven P Djordjevic
Jun 11, 2019·Frontiers in Immunology·Anneleen M F MatthijsArtur Summerfield

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