Evaluation of the embryonic preimplantation potential of human adult somatic cells via an embryo interspecies bioassay using bovine oocytes

Fertility and Sterility
Karl IllmenseePanayiotis M Zavos

Abstract

To examine the embryonic preimplantation potential of human adult somatic cells by creating interspecies embryos via somatic cell nuclear transfer (SCNT) using bovine oocytes. Prospective study. Research facility of Reprogen. Infertile couples. Enucleated bovine oocytes were fused via SCNT with either human granulosa (HG) or fibroblast (HF) cells and cultured in vitro. Polymerase chain reaction (PCR) and DNA analysis were performed on the interspecies embryos. Parthenogenetically activated embryos served as controls. Embryonic preimplantation development after interspecies SCNT. From enucleated bovine oocytes fused with HG cells (n = 48) and HF cells (n = 75), 15 HG- and 22 HF-derived embryos developed, some of which progressed to blastocysts (31.3% vs. 29.3%, respectively). The PCR and DNA analysis showed that the interspecies embryos contained human genomic DNA specific for the individual DNA profile of the HG or HF donor cells used for SCNT. In addition, both bovine- and human-specific mitochondrial DNA was detectable in the interspecies embryos up to the blastocyst stage. Parthenogenetic development was 46.8% and 64.9% for the HG and HF series, respectively. The SCNT efficiency index, defined as the ratio of SCNT and parthe...Continue Reading

References

Sep 1, 1989·Biology of Reproduction·R S PratherN L First
Mar 6, 1986·Nature·S M Willadsen
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Sims, N L First
Oct 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J FulkaR M Moor
Aug 1, 1997·Biology of Reproduction·L MengD P Wolf
Nov 6, 1998·Science·J A ThomsonJ M Jones
Dec 16, 1998·Science·Y KatoY Tsunoda
Dec 10, 1999·Nature Biotechnology·R P LanzaM D West
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·C KubotaX Yang
Nov 4, 2000·Journal of Reproduction and Fertility·Y KatoY Tsunoda
Nov 4, 2000·Nature Genetics·X C TianX Yang
Oct 10, 2001·Current Biology : CB·D Bourc'hisE Viegas-Péquignot
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·W DeanW Reik
Mar 19, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·K S O'Shea
May 3, 2002·European Journal of Biochemistry·Paul A Wade, Nobuaki Kikyo
May 22, 2002·Biology of Reproduction·Norikazu MiyashitaTakashi Nagai
Aug 31, 2002·Molecular Reproduction and Development·Fuliang DuXiangzhong Yang
Feb 18, 2003·Molecular Reproduction and Development·Kumiko TakedaHirofumi Hanada
Mar 19, 2003·Differentiation; Research in Biological Diversity·Wei ShiEckhard Wolf
Apr 12, 2003·Science·Calvin SimerlyGerald Schatten
May 10, 2003·Nature Cell Biology·A John ClarkWei Cui
Jul 4, 2003·Molecular Reproduction and Development·Cai-Xia YangDa-Yuan Chen
Jul 31, 2003·Reproduction in Domestic Animals = Zuchthygiene·S Hiendleder, E Wolf
Oct 17, 2003·Biology of Reproduction·Irina LagutinaCesare Galli

❮ Previous
Next ❯

Citations

Feb 10, 2011·Zygote : the Biology of Gametes and Early Embryos·So Gun HongByeong Chun Lee
May 11, 2011·Zygote : the Biology of Gametes and Early Embryos·Dae Kee KwonByeong Chun Lee
Feb 28, 2007·Nature Genetics·Xiangzhong YangTeruhiko Wakayama
Dec 17, 2008·The American Journal of Bioethics : AJOB·Francoise Baylis
Sep 17, 2013·Cellular Reprogramming·Irina LagutinaCesare Galli
Feb 18, 2011·The Journal of Reproduction and Development·Sumeth ImsoonthornruksaRangsun Parnpai
Sep 13, 2007·Stem Cell Reviews·Vanessa J Hall, Miodrag Stojkovic
Sep 13, 2007·Stem Cell Reviews·R Tayfur TecirliogluAlan O Trounson
Apr 22, 2011·Cell Proliferation·J Suaudeau
Apr 14, 2016·In Vitro Cellular & Developmental Biology. Animal·Uma Mahesh YelisettiSambasiva Rao Brahmasani
Mar 29, 2008·Cell Stem Cell·Zeki BeyhanJose B Cibelli
Jan 1, 2010·Human Reproduction and Genetic Ethics·David Albert Jones
Mar 6, 2012·Cellular Reprogramming·S Morteza HosseiniMohammad H Nasr-Esfahani
Jul 3, 2013·Cellular Reprogramming·Mukesh Kumar GuptaSang Jun Uhm
May 11, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Dasari AmarnathKeith H S Campbell
Jun 10, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Irina LagutinaCesare Galli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.