Evaluation of the enzymatic activity and stability of commercial bromelain incorporated in topical formulations

International Journal of Cosmetic Science
C B LourençoP G Mazzola

Abstract

Bromelain is a mixture of proteolytic enzymes found in various tissues of the pineapple plant (Ananas comosus) and other species of Bromeliaceae. Owing to its proteolytic activity, bromelain has been used in the food, medical, pharmaceutical and cosmetic industries, for its cell renewal, anti-ageing, whitening and anti-cellulite properties. This study evaluated the stability of bromelain (commercial powder) incorporated in topical formulations. Bromelain was incorporated at three concentrations, 0.5%, 1.0% and 2.0%, in oil-in-water emulsion and gel, and stored for six months at varying stress conditions. Stability was accessed by measuring the changes in the protein content, enzymatic activity, viscosity, rheology, pH and colour of the selected formulations. The colour of all the samples changed after 180 days of incubation, indicating the concentration-dependence and temperature-sensitive nature of these formulations. No relationship was observed between the changes in the pH, temperature and luminosity exposure in all the samples. Gels proved to be the least preferred base for incorporation of bromelain for use as a topical formulation, owing to its inability to maintain the integrity of bromelain, thereby affecting the formu...Continue Reading

References

Jun 1, 1992·Burns : Journal of the International Society for Burn Injuries·J G MillerD A Burd
Jun 1, 1980·Journal of Biochemical and Biophysical Methods·R H Kottel, W C Hanford
Feb 24, 2001·Journal of the European Academy of Dermatology and Venereology : JEADV·A B Rossi, A L Vergnanini
Mar 16, 2001·Trends in Biotechnology·J M Sanchez-Ruiz, G I Makhatadze
Oct 2, 2001·Cellular and Molecular Life Sciences : CMLS·H R Maurer
Dec 31, 1960·Lancet·S L DUNCANH R MACLENNAN
Feb 16, 2005·International Immunopharmacology·Laura P HaleCindy L James
May 21, 2008·International Journal of Cosmetic Science·F TerranovaH Maibach
Aug 25, 2009·Cancer Letters·Katya ChobotovaFadzilah Adibah Abdul Majid
Mar 20, 1947·The Journal of General Physiology·M Kunitz
Mar 15, 2011·The Journal of Trauma·Wei HuJian-Min Wang

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Citations

May 4, 2018·Phytotherapy Research : PTR·Janaína Artem AtaidePriscila Gava Mazzola
Sep 3, 2020·Journal of Food Biochemistry·Mulkah Olufemi Ajagun-Ogunleye, Osaretin Albert Taiwo Ebuehi

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