Evaluation of the Escherichia coli HK82 and BS87 strains as tools for AlkB studies

DNA Repair
Damian MieleckiElzbieta Grzesiuk

Abstract

Within a decade the family of AlkB dioxygenases has been extensively studied as a one-protein DNA/RNA repair system in Escherichia coli but also as a group of proteins of much wider functions in eukaryotes. Two strains, HK82 and BS87, are the most commonly used E. coli strains for the alkB gene mutations. The aim of this study was to assess the usefulness of these alkB mutants in different aspects of research on AlkB dioxygenases that function not only in alkylated DNA repair but also in other metabolic processes in cells. Using of HK82 and BS87 strains, we found the following differences among these alkB(-) derivatives: (i) HK82 has shown more than 10-fold higher MMS-induced mutagenesis in comparison to BS87; (ii) different specificity of Arg(+) revertants; (iii) increased induction of SOS and Ada responses in HK82; (iv) the genome of HK82, in comparison to AB1157 and BS87, contains additional mutations: nalA, sbcC, and nuoC. We hypothesize that in HK82 these mutations, together with the non-functional AlkB protein, may result in much higher contents of ssDNA, thus higher in comparison to BS87 MMS-induced mutagenesis. In the light of our findings, we strongly recommend using BS87 strain in AlkB research as HK82, bearing severa...Continue Reading

References

Apr 30, 2003·Environmental and Molecular Mutagenesis·Celina JanionElzbieta Grzesiuk
Jun 27, 2003·DNA Repair·John C ConnellyDavid R F Leach
May 19, 2009·Environmental and Molecular Mutagenesis·Jadwiga NieminuszczyElzbieta Grzesiuk
Feb 1, 2012·PloS One·Damian MieleckiElżbieta Grzesiuk
Dec 12, 2012·Science·Abu Amar M Al MamunSusan M Rosenberg
Aug 7, 2013·Antimicrobial Agents and Chemotherapy·Sandra Aedo, Yuk-Ching Tse-Dinh
May 9, 2014·FEMS Microbiology Letters·Damian Mielecki, Elżbieta Grzesiuk
Nov 9, 2014·The Journal of Biological Chemistry·Gururaj ShivangeRoy Anindya
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·Damian MieleckiElżbieta Grzesiuk
Mar 24, 2015·DNA Repair·Daria ZdżalikBarbara Tudek

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Citations

Mar 27, 2016·The Journal of Biological Chemistry·Qi LiCai-Guang Yang

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