Abstract
Podosomes are submicron adhesive and mechanosensitive structures formed by macrophages, dendritic cells and osteoclasts that are capable of protruding into the extracellular environment. Built of an F-actin core surrounded by an adhesion ring, podosomes assemble in a network interconnected by acto-myosin cables. They have been shown to display spatiotemporal instability as well as protrusion force oscillations. To analyse the entire population of these unstable structures, we have designed an automated multi-particle tracking adapted to both topographical and fluorescence data. Here we describe in detail this approach and report the measurements of individual and collective characteristics of podosome ensembles, providing an integrated picture of their activity from the complementary angles of organisation, dynamics, mobility and mechanics. We believe that this will lead to a comprehensive view of podosome collective behaviour and deepen our knowledge about the significance of mechanosensing mediated by protrusive structures.
References
Feb 29, 1980·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D Marr, E Hildreth
Feb 18, 2003·Molecular Biology of the Cell·Olivier DestaingFrédéric Bard
May 21, 2003·The Journal of Cell Biology·James G EvansPaul Matsudaira
Jul 3, 2003·Trends in Cell Biology·Stefan Linder, Martin Aepfelbacher
Mar 21, 2006·European Journal of Cell Biology·Pierre JurdicOlivier Destaing
Mar 24, 2006·Molecular Biology of the Cell·Petra KoppStefan Linder
Feb 1, 2007·PloS One·Chen LuxenburgLia Addadi
Dec 19, 2007·Cell and Tissue Research·Toshitaka AkisakaKeiko Takama
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Apr 9, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·C Vonesch, M Unser
Apr 2, 2010·Journal of Cell Science·Christian Gawden-BoneJohn Lucocq
Nov 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anna LabernadieGuillaume M Charrière
Feb 14, 2012·Journal of Cell Science·Chen LuxenburgBenjamin Geiger
Jan 26, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Marjolein B M MeddensKoen van den Dries
Jan 31, 2013·Nature Communications·K van den DriesA Cambi
May 3, 2013·Molecular Biology of the Cell·K van den DriesA Cambi
Jan 21, 2014·Nature Methods·Nicolas ChenouardErik Meijering
Feb 14, 2014·PloS One·Marie WaldeSusan Cox
Oct 17, 2014·Immunological Reviews·Isabelle Maridonneau-Parini
Nov 12, 2014·Nature Communications·Anna LabernadieIsabelle Maridonneau-Parini
Mar 21, 2015·ACS Nano·Amsha ProagRenaud Poincloux
Citations
Sep 1, 2015·Methods : a Companion to Methods in Enzymology·Michael J SiedlikCeleste M Nelson
Feb 21, 2016·Methods : a Companion to Methods in Enzymology·Nicolas BorghiChristophe Lavelle
Mar 30, 2017·ACS Nano·Anaïs BouissouRenaud Poincloux
Feb 29, 2020·Current Opinion in Hematology·Florian AlonsoElisabeth Génot
Mar 11, 2020·Journal of Cell Science·Robert HerzogStefan Linder
Oct 20, 2019·Nature Communications·Roxanne GlazierKhalid Salaita
Dec 15, 2019·Journal of Cell Science·Koen van den DriesRenaud Poincloux
May 19, 2021·Cellular Signalling·Yasaswi Gayatri Mishra, Bramanandam Manavathi