Evaluation of the free-radical-scavenging activity of diclofenac acid on the free-radical-induced haemolysis of human erythrocytes

The Journal of Pharmacy and Pharmacology
You-Zhi Tang, Zai-Qun Liu

Abstract

Free-radical-induced peroxidation in-vivo is regarded as the aetiology of some diseases and free-radical-scavenging drugs, also called antioxidants (AH), have been widely used to overcome oxidative stress. An in-vitro experimental method, 2,2'-azobis(2-amidinopropane hydrochloride) (AAPH)-induced haemolysis of human erythrocytes can be applied to assess the free-radical-scavenging activity of a drug. The major objectives of this work were focused on three aspects. Firstly, introduction of the chemical kinetic deduction of free-radical-initiating reaction to AAPH-induced haemolysis of human erythrocytes, by which the number of free radicals trapped by an antioxidant, n, can be obtained after finding the quantitative relationship between the inhibition period (t(inh)) and the concentration of the antioxidant, t(inh) = (n/Ri) [AH]. Ri, the free-radical-initiating rate, was initially confirmed by using alpha-tocopherol (VE) whose n was taken as 2. Secondly, the free-radical-scavenging activity of diclofenac acid (DaH) and its sodium salt (DaNaH) was assessed. It has been found that DaH and DaNaH protect human erythrocytes against AAPH-induced haemolysis dose-dependently. In particular, the n values of DaH and DaNaH (4.96 and 3.60) ...Continue Reading

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Citations

Apr 11, 2009·Drug Development and Industrial Pharmacy·C Rojo-VergaraM J Alvarez-Figueroa
Jan 17, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Juan Antonio López-VillodresJosé Antonio González-Correa
Nov 27, 2007·Journal of Neurochemistry·Elisaveta MilushevaBeáta Sperlágh
May 16, 2014·The Journal of Pharmacy and Pharmacology·Amit AlexanderShailendra Saraf
May 8, 2008·Journal of Cardiovascular Pharmacology·Stephen Fernandes RodriguesZuleica B Fortes
Aug 26, 2009·Journal of Biochemical and Molecular Toxicology·Feng Zhao, Zai-Qun Liu
Aug 26, 2009·Journal of Biochemical and Molecular Toxicology·Guo-Xiang LiZai-Qun Liu
Nov 6, 2008·Journal of Agricultural and Food Chemistry·You-Zhi Tang, Zai-Qun Liu
Jan 24, 2007·Bioorganic & Medicinal Chemistry·You-Zhi Tang, Zai-Qun Liu
Oct 14, 2010·Chemical Reviews·Zai-Qun Liu

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