Evaluation of the genotoxic potential of the Casearia sylvestris extract on HTC and V79 cells by the comet assay

Toxicology in Vitro : an International Journal Published in Association with BIBRA
E L MaistroM S Mantovani

Abstract

Casearia sylvestris is common in tropical America growing wild in Brazil in the states of Amazonas and São Paulo. Its leaves are used in Brazilian folk medicine for several diseases. The present investigation was carried out to examine the genotoxic effects of a C. sylvestris crude ethanolic extract on Hepatoma Tissue Culture (HTC cells) of Rattus norvegicus and Chinese hamster V79 cells in culture, using the comet assay. For the genotoxic evaluation the cells were treated with three different concentrations (0.5, 1 and 2 mg/ml) of extract prepared from a 25 mg/ml aqueous solution. The positive control was cyclophosphamide for HTC cells and methyl methanesulfonate for V79 cells. The duration of the treatment was 2 h. The results showed that the extract of C. sylvestris presented no genotoxic effects and not modified effect inducing DNA damage by alkylating agents cyclophosphamide and methyl methanesulfonate in HTC and V 79 cells respectively.

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Citations

May 11, 2013·Genetics and Molecular Research : GMR·V R MoreiraS R F Pereira
Apr 3, 2007·Journal of Ethnopharmacology·E S de MattosA P Piovezan
Apr 5, 2008·Phytotherapy Research : PTR·M Cintra-FrancischinelliY Oshima-Franco
Mar 19, 2016·Journal of Natural Products·Rodrigo Moreira da SilvaVeronika Butterweck
Aug 25, 2009·Journal of Applied Toxicology : JAT·Carla Carolina MunariDenise Crispim Tavares
Sep 12, 2006·Journal of Ethnopharmacology·Michele Luciane DickelMara Rejane Ritter
Dec 4, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Aline M PrietoChristiane P Soares
Jun 16, 2016·Genetics and Molecular Biology·Leonardo Campos ZanelattoMário Sérgio Mantovani
Jul 23, 2015·Journal of Applied Oral Science : Revista FOB·Raquel Zanin MidenaMarco Antonio Hungaro Duarte
Dec 19, 2013·Anais Da Academia Brasileira De Ciências·Josiane C ClaudinoAndré G dos Santos
Dec 31, 2018·Journal of Ethnopharmacology·Larissa SpósitoTaís Maria Bauab
Sep 8, 2010·Chemico-biological Interactions·Paulo M Pinheiro FerreiraCláudia Pessoa

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