Evaluation of the genotoxicity of stevioside and steviol using six in vitro and one in vivo mutagenicity assays

Mutagenesis
M MatsuiT Sofuni

Abstract

Stevioside, a constituent of Stevia rebaudiana, is commonly used as a non-caloric sugar substitute in Japan. The genetic toxicities of stevioside and its aglycone, steviol, were examined with seven mutagenicity tests using bacteria (reverse mutation assay, forward mutation assay, umu test and rec assay), cultured mammalian cells (chromosomal aberration test and gene mutation assay) and mice (micronucleus test). Stevioside was not mutagenic in any of the assays examined. The aglycone, steviol, however, produced dose-related positive responses in some mutagenicity tests, i.e. the forward mutation assay using Salmonella typhimurium TM677, the chromosomal aberration test using Chinese hamster lung fibroblast cell line (CHL) and the gene mutation assay using CHL. Metabolic activation systems containing 9000 g supernatant fraction (S9) of liver homogenates prepared from polychlorinated biphenyl or phenobarbital plus 5,6-benzoflavone-pretreated rats were required for mutagenesis and clastogenesis. Steviol was weakly positive in the umu test using S.typhimurium TA1535/pSK1002 either with or without the metabolic activation system. Steviol, even in the presence of the S9 activation system, was negative in other assays, i.e. the reverse ...Continue Reading

Citations

Oct 17, 2003·Phytochemistry·Jan M C Geuns
Dec 31, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E KoyamaM Ui
May 10, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Eriko KoyamaMichio Ui
Sep 10, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jan M C GeunsBert Driessen
Jan 20, 2010·Clinical and Experimental Pharmacology & Physiology·Hongping JinAndrew K Davey
Jul 20, 2002·Chemical & Pharmaceutical Bulletin·Tadamasa TeraiTetsuhito Hayashi
Jan 23, 2014·Applied Biochemistry and Biotechnology·Pratibha GuptaSanjay Saxena
Dec 8, 2009·International Journal of Food Sciences and Nutrition·S K GoyalR K Goyal
Oct 2, 2012·Colloids and Surfaces. B, Biointerfaces·Indu BarwalSubhash C Yadav
May 12, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Lonnie D Williams, George A Burdock
Nov 13, 2008·Pharmacology & Therapeutics·Varanuj Chatsudthipong, Chatchai Muanprasat
Jun 17, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D J Brusick
Jan 9, 2007·Phytochemistry·Li-Ming YangShwu-Jiuan Lin
Dec 26, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A P M NunesA Caldeira-de-Araújo
Jan 8, 2011·Archiv der Pharmazie·Goutam BrahmachariArun K Brahmachari
Nov 5, 2002·The Journal of Clinical Pediatric Dentistry·Michael W Roberts, J Timothy Wright
Oct 30, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J D UrbanD J Brusick
Sep 14, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pablo Anselmo GarcíaRonan Batista
Jul 26, 2017·Drug and Chemical Toxicology·Aslı UçarMustafa Sefa Kılıç
Jan 13, 2005·The Journal of Pharmacology and Experimental Therapeutics·Chutima SrimaroengJohn B Pritchard
Jan 22, 2020·Drug and Chemical Toxicology·Şemsi Gül YılmazSerkan Yılmaz
Jun 29, 2006·Molecular and Cellular Biochemistry·A P M NunesA Caldeira-de-Araujo
Aug 14, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Nikolai BondarevAlexander Nosov
Sep 3, 2020·International Journal of Environmental Research and Public Health·Ariana SaraivaAntónio Raposo
Feb 16, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G A ChappellD S Wikoff
Nov 12, 2010·Journal of Agricultural and Food Chemistry·Ursula Wölwer-RieckAndreas Wawrzun
Aug 30, 2021·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Isabel A LeaDaniele S Wikoff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.