Evaluation of the HPV 18 antibody response in Gardasil® vaccinees after 48 mo using a pseudovirion neutralization assay

Human Vaccines & Immunotherapeutics
Christine RobertsJanine Bryan

Abstract

The pseudo-neutralization assay (PsV) described in the current report allows for the creation of HPV18 pseudovirions in order to evaluate whether the antibody responses elicited following vaccination with Gardasil(®) are sufficient to neutralize the activity of these pseudovirions in vitro. The PsV assay evaluates a broader antibody response than the HPV competitive Lumniex Immunoassay (cLIA), which monitors the presence of a single neutralizing epitope. We employed two different approaches to the HPV18-PsV assay: one using standard dilutions of heat inactivated serum from vaccinated subjects, as is typically reported in the literature, and the other using heat inactivated serum from which IgG antibodies were purified and quantitated as an attempt to reduce assay background and achieve a level of quantitation greater than that afforded through simple dilution. Here, we show that after 48 mo, Gardasil(®) vaccinated subjects from three groups defined by HPV 18 cLIA titer have detectable HPV18 neutralizing antibodies as measured by either approach in the HPV18-PsV assay. These data support the observed sustained HPV18 protection against persistent infection and disease with the absence of breakthrough cases in Gardasil(®) vaccinee...Continue Reading

Citations

Nov 26, 2015·Expert Review of Vaccines·Erna Milunka KojicSusan Cu-Uvin
Apr 12, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Erna Milunka KojicSusan Cu-Uvin
Jun 6, 2013·Nature Reviews. Clinical Oncology·Matti Lehtinen, Joakim Dillner
Feb 16, 2021·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Joel M PalefskyNagalingeswaran Kumarasamy
Dec 22, 2016·ACS Applied Materials & Interfaces·Shi JinYuqing Wu

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