PMID: 2504805Jun 1, 1989Paper

Evaluation of the human gonadotroph free alpha-subunit secretory pools by administration of gonadotropin hormone-releasing hormone into normal subjects at different phases of the ovarian cycle

Journal of Endocrinological Investigation
M S BalinR Benveniste

Abstract

The specificity of a monoclonal antibody RIA for the measurement of free alpha-subunit in plasma is presently documented. This RIA was used to explore the pituitary gonadotroph free alpha-subunit reserve in normal ovulating women stimulated with gonadotropin hormone-releasing hormone (GnRH). RIA specificity was established by means of competitive inhibition curves with various glycoprotein hormone preparations, and Sephadex G-100 exclusion chromatography of purified hFSH (1-2), hLH (LER 960) and pituitary extract. The 3.8% and 5.5% crossreactivities of hFSH 1-2 and hLH LER 960 were shown by exclusion chromatography to result in part from free alpha-subunit contamination in these purified preparations. Pituitary extract chromatography indicated hFSH and hLH cross-reactivity below 2.5% and 1.5%, respectively. Normal females were stimulated with GnRH throughout the cycle: 3 tests were performed on Day 7, 1 test on Day 13, 16, 17 and 22, respectively, and 2 tests on Day 24. GnRH stimulation consisted of an initial 100 micrograms bolus (time 0) followed at 2 h by a 12.5 micrograms/h constant infusion, and a second 100 micrograms bolus at 5 h. In all subjects, baseline free alpha-subunit values were below 2 ng/ml. Total free alpha-su...Continue Reading

References

Apr 1, 1975·European Journal of Clinical Investigation·R BenvenisteD Rabinowitz
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