Evaluation of the immunochromatographic device for the detection of verotoxins in cultures of food materials

Biocontrol Science
Shigeko UedaManami Yamaguchi

Abstract

The immunochromatographic assay, which targets Shiga toxin 1/verotoxin 1 (VT1) and/or Shiga toxin 2/verotoxin 2 (VT2) independently with same test device, was used for easily, rapidly and specifically detecting verotoxin-producing Escherichia coli among E. coli strains from food and fecal materials. All 10 strains of VT 1 and/or VT 2- producing E. coli among E. coli isolates from various sources showed a positive reaction to VT1- or VT2- antibodies, but other gram-negative and positive bacterial species had a negative reaction. Bacterial counts of 10(8) cfu/ml in enrichment broth and food suspension were required for the detection of VT-producing E. coli. The IC assay described here could detect easily and specifically the verotoxin-producing E. coli within 20 min by pure culture.

References

May 6, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cheleste M Thorpe
Sep 20, 2005·The Journal of Infectious Diseases·John T BrooksNancy A Strockbine
Feb 5, 2011·Emerging Infectious Diseases·Ursula KäppeliRoger Stephan
Jul 5, 2012·Journal of Clinical Microbiology·Flemming ScheutzAlison D O'Brien

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