PMID: 9546648Apr 18, 1998Paper

Evaluation of the impact of S-adenosylhomocysteine metabolic pools on cytosine methylation of the tobacco genome

European Journal of Biochemistry
M FojtováA Holý

Abstract

We explored the possibility that the cytosine DNA methylation might be regulated by S-adenosyl-L-methionine (AdoMet) and S-adenosyl-L-homocysteine (AdoHcy) pools in plant cells. In order to change the AdoHcy/AdoMet ratio (methylation index), (S)-9-(2,3-dihydroxypropyl)adenine was employed, a selective reversible inhibitor of cellular S-adenosyl-L-homocysteine hydrolase. Micromolar concentrations of the inhibitor increased dramatically (more than 1000-fold) intracellular AdoHcy levels (and concominantly the AdoHcy/AdoMet ratio) in tobacco TBY-2 cells. No toxic effect of the drug was observed and the cells displayed only marginal inhibition of growth at high AdoHcy levels. At near equal intracellular concentrations of AdoHcy and AdoMet, a significant reduction of cytosine methylation in transcribed (5SrDNA) and non-transcribed (HRS60, NTRS) sequences was observed. Interestingly, the CpCpG and CpApG trinucleotide targets appeared to be most sensitive to changes in the methylation index. Methylation of cytosine residues at CpG sites was not affected even at AdoHcy/AdoMet ratio of > 10. These results support the possible regulation of DNA methylation via AdoHcy/AdoMet metabolic pathways in plant cells.

Citations

Oct 29, 2005·Planta·Jinsuk J LeeZ Jeffrey Chen
Apr 20, 2001·The Plant Journal : for Cell and Molecular Biology·P RanochaA D Hanson
Sep 24, 2004·Molecular Plant-microbe Interactions : MPMI·Gitta L CoakerDavid M Francis
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Andrew D Hanson, Sanja Roje
Jan 8, 2014·BMC Genetics·Jaroslav Fulneček, Aleš Kovařík
Dec 18, 2013·Nucleic Acids Research·Anna OgrockáMiloslava Fojtová
Nov 23, 2006·Journal of Experimental Botany·Jae Kwang KimAkio Kobayashi
Jan 9, 2008·The Journal of Biological Chemistry·Camilo A MoncadaSalim Merali
Sep 28, 2004·The Plant Journal : for Cell and Molecular Biology·Hangning ZhangAndreas Graner
Mar 23, 2020·Molecular Metabolism·Christian LindermayrMartin Groth

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