Abstract
We explored the possibility that the cytosine DNA methylation might be regulated by S-adenosyl-L-methionine (AdoMet) and S-adenosyl-L-homocysteine (AdoHcy) pools in plant cells. In order to change the AdoHcy/AdoMet ratio (methylation index), (S)-9-(2,3-dihydroxypropyl)adenine was employed, a selective reversible inhibitor of cellular S-adenosyl-L-homocysteine hydrolase. Micromolar concentrations of the inhibitor increased dramatically (more than 1000-fold) intracellular AdoHcy levels (and concominantly the AdoHcy/AdoMet ratio) in tobacco TBY-2 cells. No toxic effect of the drug was observed and the cells displayed only marginal inhibition of growth at high AdoHcy levels. At near equal intracellular concentrations of AdoHcy and AdoMet, a significant reduction of cytosine methylation in transcribed (5SrDNA) and non-transcribed (HRS60, NTRS) sequences was observed. Interestingly, the CpCpG and CpApG trinucleotide targets appeared to be most sensitive to changes in the methylation index. Methylation of cytosine residues at CpG sites was not affected even at AdoHcy/AdoMet ratio of > 10. These results support the possible regulation of DNA methylation via AdoHcy/AdoMet metabolic pathways in plant cells.
Citations
Oct 29, 2005·Planta·Jinsuk J LeeZ Jeffrey Chen
Jan 29, 2011·Molecular Genetics and Genomics : MGG·Jaroslav FulnečekAleš Kovařík
Aug 26, 2011·Plant Molecular Biology·Eva MajerováJiří Fajkus
Jul 24, 2008·Heredity·A KovarikH Nybom
Jul 30, 2009·Heredity·L KhaitováA Kovarík
Apr 20, 2001·The Plant Journal : for Cell and Molecular Biology·P RanochaA D Hanson
Sep 24, 2004·Molecular Plant-microbe Interactions : MPMI·Gitta L CoakerDavid M Francis
Jan 22, 2005·The Plant Cell·Pedro S C F RochaIan Furner
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Andrew D Hanson, Sanja Roje
Sep 5, 2006·Genetics·Lori MullJudith Bender
Oct 17, 2013·International Journal of Molecular Sciences·Zujun YangEviatar Nevo
Jan 8, 2014·BMC Genetics·Jaroslav Fulneček, Aleš Kovařík
Dec 18, 2013·Nucleic Acids Research·Anna OgrockáMiloslava Fojtová
Dec 17, 2008·The New Phytologist·Valérie Van WilderStéphane Ravanel
Oct 2, 2012·Physiologia Plantarum·Bram Van de PoelAnnemie H Geeraerd
Nov 26, 2015·Molecular Genetics and Genomics : MGG·Jaroslav Fulneček, Roman Matyášek
Nov 23, 2006·Journal of Experimental Botany·Jae Kwang KimAkio Kobayashi
Feb 9, 2000·FEBS Letters·A KovaríkA Depicker
Jan 9, 2008·The Journal of Biological Chemistry·Camilo A MoncadaSalim Merali
Sep 28, 2004·The Plant Journal : for Cell and Molecular Biology·Hangning ZhangAndreas Graner
Nov 17, 2019·The Plant Journal : for Cell and Molecular Biology·Anna NowickaAles Pecinka
Mar 23, 2020·Molecular Metabolism·Christian LindermayrMartin Groth
Oct 29, 2000·Archives of Biochemistry and Biophysics·L M MurfittN Dudareva
Aug 7, 2021·Antioxidants·Eva Esther RudolfChristian Lindermayr