Evaluation of the intestinal current measurement method as a diagnostic test for cystic fibrosis

Pediatric Pulmonology
Malena Cohen-CymberknohMichael Wilschanski

Abstract

The sweat test and nasal potential difference measurement are now established tools in the diagnostic work up of cystic fibrosis (CF). Intestinal current measurement (ICM) is under consideration as an aid in the diagnosis of CF especially in young children. The aim of this study is to evaluate the diagnostic reliability of ICM. Rectal biopsies were obtained from three groups: CF patients, controls, and patients who were suspected for CF. ICMs were performed by mounting the rectal biopsy in an Ussing chamber and sequentially adding secretagogues while recording current changes. Twenty-one CF patients (aged 3.0 ± 3.8 years) and 16 controls (aged 15.6 ± 15.1 years) were examined and have remarkably different results (presented as µA/cm(2) ): carbachol 16.3 ± 6.9, histamine 13.2 ± 8.9, and cAMP/forskolin 4.8 ± 4.0 for control group and carbachol -1.5 ± 5.3 (P < 0.0001), histamine -1.5 ± 3.1 (P < 0.0001), and cAMP/forskolin 0.36 ± 0.67 (P < 0.0001) for the CF group. Our suggested reference values are: +5.40, +3.52, +2.19 for carbachol, histamine, and cAMP/forskolin, respectively. The combination parameter (the arithmetic sum of carbachol, histamine, and cAMP/forskolin) of +7.19 differentiates normal from abnormal (ROC curve analysis...Continue Reading

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Citations

Jul 17, 2014·American Journal of Respiratory and Critical Care Medicine·Susan E BirketSteven M Rowe
Feb 10, 2016·Clinics in Chest Medicine·John Brewington, J P Clancy
Feb 3, 2015·Pediatric Pulmonology·Harm A W M TiddensDavid A Waltz
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Oct 12, 2015·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Donald R VanDevanterPaul S Hodgkins
Jan 1, 2015·Nature Reviews. Disease Primers·Felix RatjenAndrew Bush
Jul 22, 2016·Pediatric Pulmonology·Frank A J A BodewesMicheal Wilschanski
Oct 22, 2016·Journal of Pediatric Gastroenterology and Nutrition·Michael WilschanskiSteven Freedman
Sep 1, 2018·The European Respiratory Journal·Karin M de Winter-de GrootJeffrey M Beekman
Dec 26, 2018·Frontiers in Pharmacology·Nikhil T AwatadeShafagh A Waters
Jun 3, 2021·Journal of Personalized Medicine·Simon Y GraeberMarcus A Mall

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