Evaluation of the mechanism underlying the inhibitory effect of guanidinoacetate on brain Na+, K+-ATPase activity

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
Alexandra I ZugnoA T S Wyse

Abstract

Guanidinoacetate methyltransferase deficiency (GAMT-deficiency) is an inherited neurometabolic disorder clinically characterized by epilepsy and mental retardation and biochemically by accumulation of guanidinoacetate (GAA) and depletion of creatine. Although the neurological symptoms are predominant, the pathogenesis of the brain dysfunction in this disorder is not yet established. In the present study we investigated the in vitro effect of GAA on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic plasma membrane from hippocampus of young rats. Results showed that GAA significantly inhibited Na+, K+-ATPase activity without affecting Mg2+-ATPase activity. We also evaluated the effect of glutathione (GSH), trolox, Nomega-nitro-L-arginine methyl ester (L-NAME) and taurine (Tau) on the inhibition elicited by GAA on Na+, K+-ATPase activity. GSH, trolox, L-NAME and Tau per se did not alter Na+, K+-ATPase activity. However, L-NAME and taurine prevented the inhibitory effect of GAA on this enzyme activity. Our findings suggest that the inhibition of Na+, K+-ATPase activity caused by GAA is possibly mediated by nitric oxide (NO) formation and/or synaptic membrane alteration. The present data may contribute to the understanding of th...Continue Reading

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Citations

Sep 22, 2007·Journal of Inherited Metabolic Disease·A LatiniM Wajner
Mar 18, 2009·Brain & Development·Neil Gordon
Dec 9, 2010·Human Mutation·Patricia AlcaidePilar Rodríguez-Pombo
Nov 22, 2013·Alcoholism, Clinical and Experimental Research·Kusum K KharbandaDean J Tuma
Oct 9, 2015·Amino Acids·Sergej M Ostojic
Mar 18, 2008·Neurochemical Research·Alexandra I ZugnoAngela T S Wyse
Feb 8, 2008·Neurochemical Research·Roselia Maria SpanevelloMaria Rosa Chitolina Schetinger
Feb 3, 2007·Neurochemical Research·Alexandra I ZugnoAngela T S Wyse

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