Evaluation of the Microhaplotypes panel for DNA mixture analyses

Forensic Science International. Genetics
Peng ChenFeng Chen

Abstract

The identification of a suspect in a DNA mixture typed with the standard short tandem repeat polymorphism (STR) kits has faced challenges. Several improved methods or technologies have been introduced to address this issue. However, some complex situations in the process remain elusive. In the present study, we presented a panel of 26 tiny microhaplotypes, each generating a relatively high (>3.0) effective number of alleles (Ae) and possessing low (<50 bp) sequence lengths. The average Ae and heterozygosity values among the 9 populations of 26 microhaps were in ranges from 2.60 to 4.54 and 0.59 to 0.96, respectively. Power of discrimination and power of exclusion values were ranged from 0.49 to 0.87 and 0.29 to 0.94, respectively. Significant positive correlations have been found between Ae values and heterozygosity (r = 0.43, p = 0.02) or power of discrimination values (r = 0.55, p = 0.003), respectively. The cumulative probability of detecting a mixture of two unrelated individuals could reach 0.9999998 when using a panel of 26 microhaps with Ae = 3. We further tested the panel by using massively parallel sequencing, and 14 out of 26 microhaps were successfully genotyped in a single multiplex system. 60 unrelated Chinese Han ...Continue Reading

Citations

Dec 18, 2018·Electrophoresis·Jing ZhuLin Zhang
Jun 17, 2020·Royal Society Open Science·Aliye KureshiLagabaiyila Zha
Feb 14, 2019·International Journal of Legal Medicine·Lindsay BennettDaniele Podini
Jun 25, 2018·International Journal of Legal Medicine·Peng ChenFeng Chen
Jan 28, 2021·International Journal of Legal Medicine·Riga WuHongyu Sun
Jan 12, 2020·Science & Justice : Journal of the Forensic Science Society·Agnieszka KuffelNiamh Nic Daeid
Feb 8, 2021·Forensic Science International : Synergy·Yingai ZhangShufang Zhang
Feb 23, 2021·Frontiers in Genetics·Hui JianWeibo Liang
Oct 17, 2021·International Journal of Legal Medicine·Xingchun ZhaoZichun Hua
Oct 14, 2021·Human Genetics·Andrew J PakstisKenneth K Kidd

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