Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates

The New England Journal of Medicine
Kizzmekia S CorbettBarney S Graham

Abstract

Vaccines to prevent coronavirus disease 2019 (Covid-19) are urgently needed. The effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines on viral replication in both upper and lower airways is important to evaluate in nonhuman primates. Nonhuman primates received 10 or 100 μg of mRNA-1273, a vaccine encoding the prefusion-stabilized spike protein of SARS-CoV-2, or no vaccine. Antibody and T-cell responses were assessed before upper- and lower-airway challenge with SARS-CoV-2. Active viral replication and viral genomes in bronchoalveolar-lavage (BAL) fluid and nasal swab specimens were assessed by polymerase chain reaction, and histopathological analysis and viral quantification were performed on lung-tissue specimens. The mRNA-1273 vaccine candidate induced antibody levels exceeding those in human convalescent-phase serum, with live-virus reciprocal 50% inhibitory dilution (ID50) geometric mean titers of 501 in the 10-μg dose group and 3481 in the 100-μg dose group. Vaccination induced type 1 helper T-cell (Th1)-biased CD4 T-cell responses and low or undetectable Th2 or CD8 T-cell responses. Viral replication was not detectable in BAL fluid by day 2 after challenge in seven of eight animals in both vacci...Continue Reading

References

Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Boyd YountRalph S Baric
Sep 18, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Katalin KarikóDrew Weissman
Dec 15, 2011·The Journal of Molecular Diagnostics : JMD·Fay WangYuling Luo
Jul 28, 2013·Biostatistics·Greg FinakRaphael Gottardo
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Trevor ScobeyRalph S Baric
Sep 30, 2016·Scientific Reports·April M KillikellyBarney S Graham
May 2, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kapil BahlGiuseppe Ciaramella
Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jesper PallesenJason S McLellan
May 10, 2018·The Journal of Experimental Medicine·Norbert PardiDrew Weissman
Nov 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Guillaume B E Stewart-JonesPeter D Kwong
Jan 27, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mitzi M DonaldsonKathryn E Foulds
Jan 30, 2019·Annual Review of Medicine·Barney S GrahamJason S McLellan
Feb 21, 2019·Molecular Therapy. Nucleic Acids·Kimberly J HassettLuis A Brito
Aug 3, 2019·Science·Michelle C CrankUNKNOWN VRC 317 Study Team
Sep 26, 2019·Cell Reports·Nianshuang WangJason S McLellan
Feb 20, 2020·The New England Journal of Medicine·Lirong ZouJie Wu
Feb 23, 2020·Science·Daniel WrappJason S McLellan
Mar 24, 2020·Nature·Ewen CallawayJeff Tollefson
Mar 29, 2020·Current Tropical Medicine Reports·Wen-Hsiang ChenMaria Elena Bottazzi
Apr 3, 2020·Nature·Roman WölfelClemens Wendtner
Apr 8, 2020·Immunity·Fatima Amanat, Florian Krammer
May 10, 2020·Science·Barney S Graham
May 21, 2020·Nature Communications·Trevor R F SmithKate E Broderick
May 22, 2020·Science·Jingyou YuDan H Barouch
May 22, 2020·Science·Abishek ChandrashekarDan H Barouch
Jun 20, 2020·Nature Medicine·Quan-Xin LongAi-Long Huang
Jul 15, 2020·The New England Journal of Medicine·Lisa A JacksonUNKNOWN mRNA-1273 Study Group

❮ Previous
Next ❯

Citations

Aug 20, 2020·The New England Journal of Medicine·Lisa A JacksonBarney S Graham
Sep 30, 2020·The New England Journal of Medicine·Evan J AndersonUNKNOWN mRNA-1273 Study Group
Sep 24, 2020·Nature·Florian Krammer
Sep 6, 2020·Disease Models & Mechanisms·Sarah R LeistDavid R Martinez
Sep 10, 2020·Experimental Hematology & Oncology·Ehtisham Ul HaqJiancheng Guo
Aug 26, 2020·Nature Medicine·Kathryn M Edwards
Sep 30, 2020·Nature Biotechnology·Laura DeFrancesco
Sep 4, 2020·Indian Journal of Pediatrics·Ashok Kumar Dutta
Oct 13, 2020·Frontiers in Microbiology·Yuki Furuse, Hitoshi Oshitani
Sep 29, 2020·Transboundary and Emerging Diseases·Saverio PaltrinieriStefania Lauzi
Oct 21, 2020·Science Translational Medicine·Barton F HaynesAnn Arvin
Sep 24, 2020·Nature·César Muñoz-FontelaDan H Barouch
Sep 15, 2020·Toxicologic Pathology·Bindu M BennetRani S Sellers
Oct 27, 2020·Chembiochem : a European Journal of Chemical Biology·Vassiliki DamakoudiClemens Richert
Nov 20, 2020·Health Affairs·A David PaltielRochelle P Walensky
Nov 29, 2020·Nature Communications·Soren AlexandersenTarka Raj Bhatta
Dec 4, 2020·Human Vaccines & Immunotherapeutics·Vipin M Vashishtha, Puneet Kumar
Oct 29, 2020·International Journal of Clinical Practice·Timothy Cardozo, Ronald Veazey
Dec 10, 2020·Nature Biomedical Engineering·Norbert Pardi, Drew Weissman
Dec 16, 2020·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Benjamin S BleierAndrew P Lane
Dec 31, 2020·The New England Journal of Medicine·Lindsey R BadenUNKNOWN COVE Study Group
Dec 9, 2020·British Journal of Clinical Pharmacology·Ester Calvo Fernández, Lucie Y Zhu
Dec 29, 2020·Advanced Drug Delivery Reviews·Jee Young ChungYoung Jik Kwon
Jan 4, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Brody H FoyCarl Britto
Dec 5, 2020·Nature·Katherine McMahanDan H Barouch
Dec 6, 2020·Medical Hypotheses·Wee Song Yeo, Qin Xiang Ng
Jan 12, 2021·The Journal of Biological Chemistry·Sameer Kumar MalladiRaghavan Varadarajan
Dec 20, 2020·Advanced Drug Delivery Reviews·Kyung Soo ParkJames J Moon
Nov 21, 2020·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Vladislav IzdaAmr H Sawalha
Jan 23, 2021·Vaccines·Alberto Cagigi, Karin Loré
Jan 27, 2021·Cells·Rameswari Chilamakuri, Saurabh Agarwal
Jan 23, 2021·Vaccines·Michael D BuschmannDrew Weissman
Jan 26, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Bryan OronskyTony R Reid
Jan 27, 2021·Cell·Alessandro Sette, Shane Crotty
Feb 6, 2021·Pharmaceutics·Basmah N AldosariAlanood S Almurshedi
Feb 11, 2021·Vaccines·Shaswath S ChandrasekarAdel M Talaat
Feb 11, 2021·Vaccines·Zekun MuDerek W Cain
Dec 6, 2020·Current Opinion in Pediatrics·Satoshi KamidaniEvan J Anderson

❮ Previous
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Datasets Mentioned

BETA
MN908947.3

Methods Mentioned

BETA
affinity purification
bronchoalveolar-lavage
enzyme-linked immunosorbent assay
ELISA
flow cytometry
PCR

Clinical Trials Mentioned

NCT00067054
NCT04342195

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