Evaluation of the necessity of induction for lactose-[15N, 15N]-ureide to study the colonic ammonia metabolism

Scandinavian Journal of Gastroenterology
Vicky De Preter, Kristin Verbeke

Abstract

Stable isotope labelled glycosyl ureides have been described as non-invasive markers for measurement of gastrointestinal processes. Lactose-[13C]-ureide is used for the evaluation of orocaecal transit time (OCTT), whereas lactose-[15N, 15N]-ureide is used to study the fate of the NH3 metabolism in the colon. Induction with unlabelled lactose ureide is necessary for the determination of the OCTT. In the present study, the effect of a preceding induction on the fate of 15NH3 in the colon was evaluated. Ten healthy volunteers performed two tests: the first test without induction, the second test one week later with induction, i.e. administration of 1 g lactose ureide the evening before the test. Each test consisted of a fractionated 24-h urine collection after the subjects had received a pancake test meal labelled with 75 mg lactose-[15N, 15N]-ureide. All samples were analysed for [15N]-content by combustion IRMS (isotope ratio mass spectrometry) and results were expressed as a percentage of the administered dose. No significant differences were found in the percentage dose 15N excreted in the different urine fractions between the test without and with preceding induction. The cumulative excretion rates of the 15N-isotope after 24...Continue Reading

References

Aug 29, 1997·Biochimica Et Biophysica Acta·F M RuemmeleM J Lentze
Nov 24, 1999·Biochimica Et Biophysica Acta·C MohrK D Wutzke
Jul 24, 2001·Rapid Communications in Mass Spectrometry : RCM·D J MorrisonL T Weaver
Jul 25, 2003·European Journal of Clinical Nutrition·D J MorrisonL T Weaver

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Citations

May 21, 2010·European Journal of Clinical Nutrition·K D Wutzke, J Mix
Feb 2, 2012·Seminars in Nuclear Medicine·Lawrence A Szarka, Michael Camilleri

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