Evaluation of the neonatal streptozotocin model of diabetes in rats: Evidence for a model of neuropathic pain

Pharmacological Reports : PR
Paulino Barragán-IglesiasVinicio Granados-Soto

Abstract

The purpose of this study was to evaluate the participation of satellite glial cells (SGC), microglia and astrocytes in a model of streptozotocin-induced diabetes initiated in neonatal rats (nSTZ) and to determine the pharmacological profile for pain relief. nSTZ was used to induce experimental diabetes. Von Frey filaments were used to assess tactile allodynia. Drugs were given by systemic administration. Western blotting and immunohistochemistry were used to determine protein expression and cellular localization. nSTZ produced mild hyperglycemia, weight loss, glucose intolerance, and reduction of nerve conduction velocity of C fibers. Moreover, nSTZ enhanced activating transcription factor 3 (ATF3) immunoreactivity in dorsal root ganglia (DRG) and sciatic nerve of adult rats. ATF3 was found in SGC (GFAP+ cells) surrounding DRG at week 16. Late changes in ATF3 immunoreactivity in DRG correlated with up-regulation of ATF3 and GFAP protein expression. nSTZ increased GFAP and OX-42 immunoreactivity and percentage of hypertrophied and ameboid microglia in the spinal dorsal horn. These changes correlated with the presence of mechanical hypersensitivity (tactile allodynia). Administration of gabapentin (30-100mg/kg, po) and metformin...Continue Reading

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Citations

Aug 5, 2020·Journal of Neurochemistry·Úrzula Franco-EnzástigaVinicio Granados-Soto
Jul 30, 2019·Journal of Diabetes Research·Martha Isela Barragán-BonillaMónica Espinoza-Rojo
May 15, 2020·Neurochemical Research·Adriana JiménezRosalinda Guevara-Guzmán
Jan 27, 2021·The Journal of Comparative Neurology·Karthik RaviArumugam R Jayakumar
Nov 13, 2020·Frontiers in Pharmacology·Guadalupe Del Carmen Baeza-FloresVinicio Granados-Soto
Jun 24, 2020·Peptides·Richard J Bodnar
Aug 28, 2021·Biomedicines·Kjeld Morten MohrChristian Bjerggaard Vaegter

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