Evaluation of the nitrate-based colorimetric method for testing the susceptibility of Mycobacterium tuberculosis to streptomycin and ethambutol in liquid cultures

The Journal of Antimicrobial Chemotherapy
Heidi SyreHarleen M S Grewal

Abstract

To evaluate the inexpensive colorimetric nitrate reductase-based antibiotic susceptibility (CONRAS) assay for testing the susceptibility of Mycobacterium tuberculosis to streptomycin and ethambutol in liquid cultures, and to compare the CONRAS test with the manual mycobacteria growth indicator tube (MGIT) test, using the radiometric BACTEC 460TB method as reference. A total of 89 M. tuberculosis isolates were tested for susceptibility to streptomycin and ethambutol using the CONRAS and manual MGIT methods and the results were compared with BACTEC 460TB. Isolates with discrepant results between the CONRAS test and BACTEC 460TB were analysed using the agar proportion method, Etest and mutation analysis of genes involved in resistance to streptomycin and ethambutol. The agreement between the CONRAS test and BACTEC 460TB was 88% for streptomycin and 84% for ethambutol. The corresponding agreement of the manual MGIT test with BACTEC 460TB was 89 and 80%, respectively. There was good agreement for streptomycin and moderate agreement for ethambutol between the CONRAS and manual MGIT tests on one hand and BACTEC 460TB on the other (CONRAS test, kappa(streptomycin) 0.74 and kappa(ethambutol) 0.59, P < 0.001; manual MGIT test, kappa(stre...Continue Reading

References

Dec 1, 1986·Antimicrobial Agents and Chemotherapy·L B HeifetsP J Lindholm-Levy
Mar 1, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·T C VictorR Warren
Feb 5, 2002·Journal of Clinical Microbiology·K A Kristian AngebySven E Hoffner
Aug 25, 2004·International Journal of Antimicrobial Agents·Ahmet Yilmaz CobanBelma Durupinar

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Citations

Mar 24, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J C PalominoF Portaels
Nov 18, 2014·Biomarkers in Medicine·Juan Carlos PalominoAnandi Martin
Jun 10, 2011·Memórias do Instituto Oswaldo Cruz·Ahmet Yilmaz CobanBelma Durupinar

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