PMID: 22576429May 12, 2012Paper

Evaluation of the nitrate reductase assay for the rapid detection of resistance to first-line medications in Mycobacterium tuberculosis strains isolated from patients in a general hospital

Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia
Maria de Fátima Filardi Oliveira MansurSilvana Spíndola de Miranda

Abstract

We compared the nitrate reductase assay with the proportion method, which is considered the gold standard, in 57 Mycobacterium tuberculosis strains isolated from patients treated at the Federal University of Minas Gerais Hospital das Clínicas, located in the city of Belo Horizonte, Brazil. For rifampin and isoniazid, the sensitivity, specificity, and accuracy of the nitrate reductase assay were all 100%, whereas they were 100%, 88.9%, and 66.7%, respectively, for streptomycin and 98.0%, 100%, and 98.2%, respectively, for ethambutol. The mean time to results was ten days. In the study sample, the nitrate reductase assay proved highly accurate and showed excellent concordance with the gold standard.

References

Sep 1, 1995·Journal of Clinical Microbiology·D M YajkoW K Hadley
Feb 5, 2002·Journal of Clinical Microbiology·K A Kristian AngebySven E Hoffner
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Juan-Carlos PalominoFrançoise Portaels
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Jan 1, 2008·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Karine O SanchotenePedro E A da Silva

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