Evaluation of the One-Step ELISA kit for the detection of buprenorphine in urine, blood, and hair specimens

Forensic Science International : Synergy
V CirimeleP Kintz

Abstract

A solid-phase enzyme immunoassay involving microtiter plates was recently proposed by International Diagnostic Systems corporation (IDS) to screen for buprenorphine in human serum. The performance of the kit led us to investigate its applicability in other biological matrices such as urine or blood, and also hair specimens. Low concentrations of buprenorphine were detected with the ELISA test and confirmed by HPLC/MS (buprenorphine concentrations measured by HPLC/MS: 0.3 ng/mL in urine, 0.2 ng/mL in blood, and 40 pg/mg in hair). The intra-assay precision values were 8.7% at 1 ng/mL of urine (n = 8), 11.5% at 2 ng/mL in serum (n = 8), and 11.5% at 250 pg/mg of hair (n = 8), respectively. The immunoassay had no cross-reactivity with dihydrocodeine, ethylmorphine, 6-monoacetylmorphine, pholcodine, propoxyphene, dextromoramide, dextrometorphan at 1 and 10 mg/L, or codeine, morphine, methadone, and its metabolite EDDP. A 1% cross-reactivity was measured for a norbuprenorphine concentration of 50 ng/mL. Finally, the immunoassay was validated by comparing authentic specimens results with those of a validated HPLC/MS method. From the 136 urine samples tested, 93 were positive (68.4%) after the ELISA screening test (cutoff: 0.5 ng/mL) a...Continue Reading

References

Oct 27, 1998·Journal of Analytical Toxicology·A TracquiB Ludes
Sep 22, 2000·Journal of Analytical Toxicology·V CirimeleB Ludes
Apr 3, 2003·Journal of Analytical Toxicology·V CirimeleB Ludes

❮ Previous
Next ❯

Citations

Jun 25, 2009·Analytical and Bioanalytical Chemistry·Javier AdrianM-Pilar Marco
Jun 1, 2005·Forensic Science International : Synergy·Marleen LaloupNele Samyn
May 24, 2006·International Journal of Toxicology·Vassiliki A BoumbaTheodore Vougiouklakis
Feb 15, 2013·Journal of Analytical Toxicology·Marcela Nogueira Rabelo AlvesAldo Polettini
Nov 29, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Nadia De GiovanniGiovanni Donzelli
Jan 25, 2014·Journal of Pharmacy & Bioallied Sciences·Noam SchildhausMichael Guarnieri
May 9, 2007·Analytical and Bioanalytical Chemistry·Frank Musshoff, Burkhard Madea
Oct 14, 2010·Journal of Pharmaceutical and Biomedical Analysis·Ioannis I PapoutsisConstantinos P Maravelias
Jan 5, 2010·The Veterinary Journal·Otto KalliokoskiKlas S P Abelson
Jan 19, 2010·Forensic Science International : Synergy·Fiorenza SvaizerMaria Pia Miozzo
Dec 1, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marta ConcheiroMarilyn A Huestis
May 15, 2013·Clinical Biochemistry·Sheng-Jin LiWei-Xian Chen
Jul 4, 2006·Forensic Science International : Synergy·Frank Musshoff, Burkhard Madea
Jun 18, 2009·Archives of Toxicology·Jelka PleadinNina Persi
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marta TikhomirovTomasz Śniegocki
Jun 15, 2005·Analytical Chemistry·T A BrettellR Saferstein
Apr 21, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fritz Pragst, Marie A Balikova

❮ Previous
Next ❯

Related Concepts

Related Feeds

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.